Anti-Neuronal acetylcholine receptor α-7, clone 6F12.2 Antibody

The protein named Neuronal acetylcholine receptor subunit alpha-7, or neuronal nicotinic acetylcholine receptor alpha7 subunit (nAChRa7) is the alpha subunit of a multi-protein, post-synaptic membrane bound ion-channel receptor complex expressed in acetylcholine responsive neurons in the brain and some immune cells. The nAChRa7 receptor belongs to the family of ligand gated ion channels. In the brain, the nAChRa7 receptor is expressed in key brain regions and it is involved in essential cognitive functions such as memory, thinking, comprehension, learning capacity, calculation, orientation, language, and judgment. The a7 subunit has also been identified as a significant mediator of nicotine interactions within the stress axis and human disease. nAChRa7 also has neuroprotective effects via multiple pathways making it a target for treatment of a number of brain disorders including Alzheimer’s disease. Finally, nAChRa7 is key to the cholinergic anti-inflammatory pathway in mediating inflammatory responses by immune cells which can aid in chronic inflammatory diseases like Atherosclerosis.

GST-tagged recombinant protein corresponding to the cytoplasmic domain of Human CHRNA7.

This Anti-Neuronal acetylcholine receptor α-7, clone 6F12.2 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of Neuronal acetylcholine receptor α-7.

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Neuronal acetylcholine receptor α-7 in 10 µg of Mouse Hypothalamus tissue lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Neuronal acetylcholine receptor α-7 in human cerebellum, cerebral cortex, and thalamic tissue.

Evaluated by Western Blotting in mouse cerebellum tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Neuronal acetylcholine receptor α-7 in 10 µg of mouse cerebellum tissue lysate.

~60 kDa observed. Uncharacterized bands may be observed in some lysates.

Format: Purified

Concentration: Please refer to lot specific datasheet.