Anti-Pink1 Antibody, clone N4/15

Mutations in the PTEN-induced kinase 1 (PINK1) gene located within the PARK6 locus on chromosome 1p35-p36 have recently been identified in patients with recessive early-onset Parkinson′s disease. Data suggests that PINK1 may be the second most common causative gene next to parkin in parkinsonism with the recessive mode of inheritance.

This antibody recognizes the cytoplasmic domain of Pink1. Other Homologies: Mouse (81% Sequence Homology) and Rat (82% Sequence Homology)

Epitope: Cytoplasmic domain

Recombinant protein corresponding to the cytoplasmic domain of human Pink1.

Anti-Pink1 Antibody, clone N4/15 is a highly specific mouse monoclonal antibody & that targets PINK1 & has been tested in western blotting.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Pink1 in 10 µg of human testis tissue lysate.

Immunohistochemistry Analysis: A representative lot detected Pink1 in rat caudate putamen tissue (Prof. J. Trimmer, University of California, Davis.).

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Pink1 in 10 µg of human skeletal muscle tissue lysate.

~60 kDa observed. Uniprot describes two isoforms at 63 kDa and 30 kDa The full length protein of Pink1 is observed at 63 kDa is the full length PINK1; however, it is proteolytically cleaved to a ~52 kDa form upon entry into the mitochondria (Jin, S. M., et al. (2010). J Cell Biol. 191(5):933-942.).

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Human skeletal muscle tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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