Anti-GABA(A)R, Alpha1 Protein Antibody, clone N95/35

The neurotransmitter GABA (gamma-aminobutyric acid) binds to specific transmembrane receptors in the plasma membrane of both pre- and postsynaptic neurons. Three general classes of GABA receptors are known: GABAA and GABAC (ionotropic) receptors and GABAB (metabotropic) receptors. The GABAA (gamma-Aminobutyric Acid type A) receptor is a multiprotein complex that acts as a ligand-gated chloride channel. These receptors are hetero-oligomers, for which 6 subunits are currently known (a, b, g, d, e and r). There are at least 6 different isoforms of the alpha subunit, and receptor subunit expression varies during development. Increased alpha1 subunit assembly in postsynaptic receptors of cerebellar inhibitory synapses is responsible for the fast inhibitory synaptic currents observed during postnatal development. Additionally, alpha1-containing GABAA receptors are important in regulating the reinforcing properties of alcohol, and expression of the alpha1 subunit increases upon chronic exposure to alcohol, making it a potential therapeutic target for alcohol dependency.

This antibody recognizes the cytoplasmic domain of GABA(A)R, Alpha1 Protein. This antibody does not demonstrate cross-reactivity against GABA(A)R2 and GABA(A)R3 (Prof. J. Trimmer, University of California, Davis).

Epitope: Cytoplasmic domain

Recombinant protein corresponding to the cytoplasmic domain of mouse GABA(A)R, Alpha1 Protein.

Immunohistochemistry Analysis: A representative lot detected GABA(A)R, Alpha1 Protein in rat hippocampus tissue (Prof. J. Trimmer, University of California, Davis).

Western Blot Analysis: A representative lot detected GABA(A)R, Alpha1 Protein in RBM membrane extracts, COS cell lysates transiently transfected with GABA(A)R, Alpha1 Protein, and in WT mice but not in GABA(A)R Alpha1 knockout mice.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

This Anti-GABA(A)R, Alpha1 Protein Antibody, clone N95/35 is validated for use in Western Blotting, IHC for the detection of GABA(A)R, Alpha1 Protein, clone N95/35.

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected GABA(A)R, Alpha1 Protein in 10 µg of mouse brain tissue lysate.

~55 kDa observed

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Mouse brain tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.