Anti-Cav3.1 Ca2+ channel Antibody, clone N178A/9

Voltage-dependent T-type calcium channel subunit alpha-1G or Voltage-gated calcium channel subunit alpha Cav3.1, like other members of the voltage-sensitive calcium channel (VSCC) family, mediate the entry of calcium ions into excitable cells. These channels regulate and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1G gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by mibefradil. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and also support calcium signaling in secretory cells and vascular smooth muscle.

Other homologies: Human (80% sequence homology).

Recombinant protein corresponding to mouse Cav3.1.

Immunohistochemistry Analysis: 1 µg/mL from a representative lot detected Cav3.1 Ca2+ channel in rat thalamus tissue.

Immunohistochemistry Analysis: A representative lot detected Cav3.1 Ca2+ in mouse thalamus, cortex, and cerebellum tissue.

Immunohistochemistry Analysis: A representative lot detected Cav3.1 Ca2+ in rat hippocampus tissue.

Western Blot Analysis: A representative lot detected Cav3.1 Ca2+ in rat brain membrane tissue lysate.

Western Blot Analysis: A representative lot detected Cav3.1 Ca2+ in rat brain membrane tissue lysate and in WT mouse brain membrane tissue lysate, but demonstrates a loss of signal in Cav3.1 Ca2+1 knockout mouse brain membrane tissue lysate.

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

This Cav3.1 Ca2+ channel antibody is validated for use in WB & IHC for the detection of the Cav3.1 Ca2+ channel protein.

Evaluated by Western Blot in mouse cerebellum tissue.

Western Blot Analysis: 1 µg/mL of this antibody from a representative lot detected Cav3.1 Ca2+ channel in 10 µg/mL of mouse cerebellum tissue.

~230 kDa observed. An uncharacterized band at ~53 kDa may be observed in some tissue lysates.

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Mouse cerebellum tissue

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.