生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
3H8, monoclonal
种属反应性
human, mouse, rat
技术
immunohistochemistry: suitable
western blot: suitable
同位素/亚型
IgG1
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... TARDBP(23435)
一般描述
TAR DNA-binding protein 43 (Tdp43) belongs to the hnRNP protein family and plays an important role in transcription, pre-mRNA splicing, mRNA stability and mRNA transport. It is involved in splicing of the apolipoprotein A-II and cystic fibrosis transmembrane gene. This protein is highly expressed in the pancreas, placenta, lung, genital tract and spleen. Mutations in Tdp43 have been associated with amyotrophic lateral sclerosis, frontotemporal dementia, Parkinson′s disease and Alzheimer′s disease.
免疫原
His-tagged recombinant protein corresponding to the N-terminus of human Tdp43.
应用
Detect TDP using this mouse monoclonal antibody, Anti-Tdp43 Antibody, clone 3H8 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Tdp43 in human frontal lobe, human cerebellum, and rat cerebellum tissue.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
质量
Evaluated by Western Blotting in mouse brain nuclear extract tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tdp43 in 10 µg of mouse brain nuclear extract tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tdp43 in 10 µg of mouse brain nuclear extract tissue lysate.
目标描述
~43 kDa observed
外形
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide with 10% glycerol.
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 2
Seiji Watanabe et al.
Cell death & disease, 11(10), 909-909 (2020-10-25)
Cytoplasmic inclusion of TAR DNA-binding protein 43 (TDP-43) is a pathological hallmark of amyotrophic lateral sclerosis (ALS) and a subtype of frontotemporal lobar degeneration (FTLD). Recent studies have suggested that the formation of cytoplasmic TDP-43 aggregates is dependent on a
Kotaro Oiwa et al.
Science advances, 9(31), eadf6895-eadf6895 (2023-08-04)
The cytoplasmic aggregation of TAR DNA binding protein-43 (TDP-43), also known as TDP-43 pathology, is the pathological hallmark of amyotrophic lateral sclerosis (ALS). However, the mechanism underlying TDP-43 cytoplasmic mislocalization and subsequent aggregation remains unclear. Here, we show that TDP-43
Jung-A A Woo et al.
Nature communications, 8, 15558-15558 (2017-06-07)
Although multiple CHCHD10 mutations are associated with the spectrum of familial and sporadic frontotemporal dementia-amyotrophic lateral sclerosis (FTD-ALS) diseases, neither the normal function of endogenous CHCHD10 nor its role in the pathological milieu (that is, TDP-43 pathology) of FTD/ALS have
Kohei Nishino et al.
Acta neuropathologica communications, 7(1), 118-118 (2019-07-28)
Intracellular mislocalization of TAR DNA-binding protein 43 (TDP-43), a nuclear DNA/RNA-binding protein involved in RNA metabolism, is a pathological hallmark of amyotrophic lateral sclerosis (ALS). Although the aggregation-prone, TDP-43 C-terminal domain is widely considered as a key component of TDP-43
Tandem detergent-extraction and immunoprecipitation of proteinopathy: Scalable enrichment of ALS-associated TDP-43 aggregates.
Evangelista, et al.
iScience, 26, 106645-106645 (2023)
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