Anti-PSMD9 Antibody, clone 16C5.1

26S proteasome non-ATPase regulatory subunit 9 (UniProt O00233; also known as 26S proteasome regulatory subunit p27, Homolog of rat Bridge 1, Proteasome macropain non-ATPase subunit 9) is encoded by the PSMD9 gene (Gene ID 5715) in human. PSMD9 is a proteasomal assembly chaperone that forms a stable subcomplex with two AAA-ATPases, PSMC3 and PSMC6, in assisting the assembly of the 20S and 19S particles to form the holo complex. PSMD9 contains a PDZ-like domain (a.a. 108-195) that is reported to interact with a short 4- to 7-amino acid linear sequence motifs (SLIMs) at the C-terminal end of its client proteins, including the RNA-binding protein hnRNPA1, the ribosomal protein S14, the growth hormone CSH1, the transcription factor E12, and the IL6 receptor. PSMD9 and hnRNPA1 interaction is found to play a role in IκBα proteasomal degradation, where hnRNPA1 functions as shuttle receptor that recruits IκBα to the proteasome for degradation.

Expected to react with spliced isoforms p27-L and p27-S, but not spliced isoform 3.

Epitope: Internal.

GST-tagged recombinant human PSMD9 internal fragment.

Anti-PSMD9 Antibody, clone 16C5.1 is an antibody against PSMD9 for use in Western Blotting, Immunohistochemistry (Paraffin).

Research Category
Neuroscience

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected PSMD9 in 10 µg of HepG2 cell lysate.
Immunohistochemistry Analysis: An 1:50 dilution from a representative lot detected PSMD9 in normal human kidney and prostate tissues, as well as human prostate cancer tissue sections.

Evaluated by Western Blotting in SHSY-5Y cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PSMD9 in 10 µg of SHSY-5Y cell lysate.

~26 kDa observed. 24.68 kDa (isoforms p27-L) and 22.76 kDa (isoforms p27-S) calculated.

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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