生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
2K9, monoclonal
分子量
calculated mol wt 139.21 kDa
observed mol wt ~140 kDa
纯化方式
using protein G
种属反应性
rat
种属反应性(根据同源性预测)
canine, porcine, feline, monkey, human
包装
antibody small pack of 100 μg
技术
immunocytochemistry: suitable
western blot: suitable
同位素/亚型
IgG3κ
表位序列
C-terminal half
Protein ID登记号
UniProt登记号
运输
ambient
靶向翻译后修饰
unmodified
基因信息
human ... HIF1AN(55662)
一般描述
Ankyrin repeat and sterile alpha motif domain-containing protein 1B (UniProt: P0C6S7; also known as Amyloid-beta protein intracellular domain-associated protein 1, AIDA-1, E2A-PBX1-associated protein, EB-1) is encoded by the Anks1b gene (Gene ID: 314721) in rat. AIDA-1 is an adaptor protein that has been implicated in Cajal body and nucleolar function. It is expressed throughout the brain and is highly expressed in the hippocampus. It is one of the most abundant proteins at postsynaptic densities where it is reported to bind to PSD95 and associates with NMDA receptors (NMDARs). In the brain, it interacts with amyloid b precursor protein and plays a role in brain development. AIDA-1 preferentially associates with GluN2B-containing NMDARs and plays a role in their transport from the endoplasmic reticulum (ER). Loss of AIDA-1 results in the accumulation of GluN2B in the ER and decreases GluN2B and increases GluN2A distribution at the synapse. However, loss of AIDA-1 does not affect AMPAR-mediated synaptic transmission or the probability of glutamate release. AIDA-1 has seven Ankyrin repeats in its N-terminal half and has two closely juxtaposed sterile alpha motif (SAM) domains (aa 810-876 and 884-949). Within the second SAM domain resides the nuclear localization signal (aa 935-938). Mutations in Anks1b gene are known to affect GluN2B function and are linked to the pathogenesis of neuropsychiatric disorders. (Ref.: Carbonell, AU., et al., (2019). Nat. Commun. 10(1); 3529; Tindi, JO., et al. (2015). J. Neurosci. 35(24); 8986- 8996).
特异性
Clone 2K9 is a mouse monoclonal antibody that detects AIDA1/ANKS1B. It targets an epitope within 10 amino acids from the C-terminal half.
免疫原
A linear peptide corresponding to 10 amino acids from the SAM1/2 domain in the C-terminal half of rat AIDA1/ANKS1B.
应用
Quality Control Testing
Evaluated by Western Blotting in Rat brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected AIDA1 in Rat brain tissue lysate (non-reduced).
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AIDA1 in rat testis tissue lysates (non-reduced).
Immunocytochemistry Analysis: A representative lot detected AIDA1 in Immunocytochemistry applications (Tindi, J.O., et al. (2015). J Neurosci. 35(24):8986-96).
Western Blotting Analysis: A representative lot detected AIDA1 in Western Blotting applications (Tindi, J.O., et al. (2015). J Neurosci. 35(24):8986-96).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Rat brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected AIDA1 in Rat brain tissue lysate (non-reduced).
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AIDA1 in rat testis tissue lysates (non-reduced).
Immunocytochemistry Analysis: A representative lot detected AIDA1 in Immunocytochemistry applications (Tindi, J.O., et al. (2015). J Neurosci. 35(24):8986-96).
Western Blotting Analysis: A representative lot detected AIDA1 in Western Blotting applications (Tindi, J.O., et al. (2015). J Neurosci. 35(24):8986-96).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-AIDA1, clone 2K9, Cat. No. MABN2638, is a mouse monoclonal antibody that detects AIDA1 and is tested for use in Immunocytochemistry and Western Blotting.
外形
Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Recommended storage: +2°C to +8°C.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
法规信息
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