Anti-Aquaporin-4 Antibody

Aquaporin-4 (UniProt: P55087; also known as AQP-4, Mercurial-insensitive water channel, MIWC, WCH4) is encoded by the AGP4 gene (Gene ID: 361) in human. Aquaporin-4 is a multi-pass membrane protein that forms a water-specific channel and serves as an osmoreceptor that regulates body water balance and mediates water flow within the central nervous system. Its extracellular domains are localized to amino acids 58-64, 137-155, and 206-231); its transmembrane domains are in amino acids 37-57, 65-85, 116-136, 156-176, 185-205, and 232-252); and the cytoplasmic domain is in amino acids 1-36, 86-115, 177-184, and 253-323). Aquaporin-4 is largely expressed in the brain and the skeletal muscle and at lower levels in heart, kidney, and lung tissue. Phosphorylation of Aquaporin-4 at Serine 180 by protein kinase C is shown to reduce conductance by about 50%, whereas phosphorylation at Serine 111 by protein kinase G in response to glutamate can increase conductance by about 40%. Two isoforms of Aquaporin-4 have been described that are produced by alternative splicing. Antibodies to Aquaporin-4 can serve as useful serum markers to distinguishing neuromyelitis optica (NMO) from multiple sclerosis. Clone mECD is shown to induce clustering and internalization of Aquporin-4 and cause its redistribution, which is also observed with NMO-IgG. Exposure to mECD antibody is also shown to cause coaggregation of astrocytic CD28B and Aquaporin-4. (Ref.: Hinson, SR et al. (2017). Proc. Natl.Acad. Sci. USA 114(21); 5491-5496).

Clone mECD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in the extracellular region.

Epitope: extracellular domain

His-tagged full-length human recombinant Aquaporin-4.

Anti-Aquaporin-4, clone mECD, Cat. No. MABN2471, is a highly specific mouse monoclonal antibody that targets Aquaporin-4 and has been tested for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, and Immunoprecipitation.

Flow Cytometry Analysis: A representative lot detected Aquaporin-4 in Flow Cytometry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).

Immunofluorescence Analysis: A representative lot detected Aquaporin-4 in Immunofluorescence applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprcipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).

Research Category
Neuroscience

Evaluated by Immunohistochemistry in rat brain frozen tissue sections.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Aquaporin-4 in Rat brain frozen tissue sections.

34.83 kDa calculated.

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.