Anti-GCAP1 Antibody, clone 6B12

Guanylyl cyclase-activating protein 1 (UniProt: P43081; also known as GCAP 1, Guanylate cyclase activator 1A) is encoded by the Guca1a (also known as Gcap, Gcap1, Guca1) gene (Gene ID: 14913) in murine species. GCAP1 is a membrane bound protein that stimulates retinal guanylyl cyclase when free calcium ions concentration is low and inhibits guanylyl cyclase when free calcium ions concentration is elevated. This Ca2+-sensitive regulation of retinal guanylyl cyclase appears to be a key event in recovery of the dark state of rod photoreceptors following light exposure. GCAP1 is present in rod and cone photoreceptor outer segments where phototransduction occurs. It binds three calcium ions (via EF-hands 2, 3 and 4) when calcium levels are high. Binds Mg2+ when calcium levels are low. Three calcium-binding sites are identified (aa 64-75; 100-111; and 144-155). GCAP1 restores the calcium sensitivity of guanylyl cyclase in a reconstituted system, and it decreases the sensitivity, time-to-peak, and recovery time of the light response following its introduction into intact rod outer segments. (Ref.: Gorczyca WA (1995). J. Biol Chem. 270(37); 22029-36; Schrem A et al. (1999). J Biol Chem. 274(10); 6244-49).

Clone 6B12 detects Guanylyl cyclase-activating protein 1 in Bovine, Human, Mouse, Porcine, and Rat. It targets an epitope within 45 amino acids from the C-terminal region.

Epitope: C-terminus

GST-fusion protein containing 45 amino acids from the C-terminal region of murine GCAP1. It does not display any homology with GCAP2.

Anti-GCAP1, clone 6B12, Cat. No. MABN2396, is a highly specific mouse monoclonal antibody that targets Guanylyl cyclase-activating protein 1 and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunofluorescence Analysis: A representative lot detected GCAP1 in Immunofluorescence applications (Zulliger, R., et. al. (2015). J Biol Chem. 290(6):3488-99).

Immunofluorescence Analysis: A 1:10 dilution from a representative lot detected GCAP1 in mouse retina.

Western Blotting Analysis: A representative lot detected GCAP1 in Western Blotting applications (Zulliger, R., et. al. (2015). J Biol Chem. 290(6):3488-99).

Immunoprecipitation Analysis: A representative lot detected GCAP1 in Immunoprecipitation applications (Zulliger, R., et. al. (2015). J Biol Chem. 290(6):3488-99).

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected GCAP1 in human retina tissue.

Research Category
Neuroscience

Evaluated by Western Blotting in C57BL/6 mouse retinal tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected GCAP1 in C57BL/6 mouse retinal tissue lysate.

23 kDa observed; 22.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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