Anti-pan Thyroid Hormone Receptor Antibody, clone N403/63

Thyroid hormone receptor alpha (UniProt P10827; also known as Nuclear receptor subfamily 1 group A member 1, V-erbA-related protein 7, EAR-7, c-erbA-1, c-erbA-alpha) is encoded by the THRA (also known as CHNG6, EAR7, ERBA, ERBA1, NR1A1, THRA1, THRA2) gene (Gene ID 7067) in human. Thyroid hormone receptors (THRs) bind to short, repeated sequences of DNA known as T3 response elements (TREs) and mediate thyroid hormone (TH) regulated gene transcriptions. THRs belong to the super family of single chain nuclear receptors with similar folded domain structures, including an N-terminal domain (A–B domain), a central DNA-binding domain (DBD), and a C-terminal ligand-binding domain (LBD). The LBD dictates ligand specificity and modulates homodimerization or heterodimerization with other family members. The DBDs of all THRs are highly homologous and each of these proteins can form heterodimers and homodimers. All THRs contain activation 1 (AF-1) region in the A–B domain; THRα1 and THRβ1-3 also contain a C-terminal AF-2 domain. THRα1 and THRα2 are truncated splice variants that do not contain AF-2 and do not bind the active TH form T3. In the absence of T3, THR recruits a co-repressor, such as N-CoR or N-CoR2.46, to the TRE sites, resulting in target transcription suppression as a result of co-repressor-mediated chromatin epigenetic modification. Upon T3 binding, the co-repressors are released, leading to the exposure of the AF-2 domain and recruitment of co-activators, such as SRC-1, that induce gene transcriptions by inducing chromatin remodeling and alter the DNA conformation.

Exprected to react with all four spliced isoforms of human thyroid hormone receptor alpha reported by UniProt (P10827). Clone N403/63 also cross-reacts with both spliced isoforms of human thyroid hormone receptor beta (UniProt P10828), but not potassium channel Kv2.1.

Recombinant full-length human thyroid hormone receptor Alpha-1 isoform.

Detect pan Thyroid Hormone Receptor using this Anti-pan Thyroid Hormone Receptor Antibody, clone N403/63 validated for use in Immunohistochemistry (Paraffin), Western Blotting.

Research Category
Neuroscience

Research Sub Category
Nuclear Receptors

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected thyroid hormone receptor in 10 µg of human and rat brain tissue lysates.
Immunohistochemistry Analysis: An 1:250 dilution from a representative lot detected thyroid hormone receptor in human cerebral cortex, mouse cerebellum, and rat kidney tissue.
Western Blotting Analysis: A representative lot detected the transient expression of c-Myc-tagged human thyroid hormone receptor alpha (isoform Alpha-1) and beta (isoforms Beta-1 & Beta-2) construts in transfected COS-7 cells (Courtesy of Professor. James S. Trimmer, UC Davis, CA, USA).
Immunohistochemistry Analysis: A representative lot detected thyroid hormone receptor immunoreactivity in rat whole brain sections (Courtesy of Professor. James S. Trimmer, UC Davis, CA, USA).

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Thyroid Hormone Receptor in 10 µg of mouse brain tissue lysate.

~70 kDa observed. Target band appears larger than the calculated molecular weights of 46.81 kDa (isoform Alpha-1), 54.81 kDa (isoform Alpha-2), 50.75 kDa (isoform Alpha-3), 50.47 kDa (isoform Alpha-4), 52.79 kDa (isoform Bet-1), 54.45 kDa (isoform Beta-2).

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.