Anti-TTBK1 Antibody, clone F287-1.1-1E9

Tau-tubulin kinase 1 (EC 2.7.11.1; UniProt Q5TCY1; also known as Brain-derived tau kinase) is encoded by the TTBK1 (also known as BDTK, KIAA1855) gene (Gene ID 84630) in human. Tau-tubulin kinase 1 and 2 (TTBK1 and TTBK2) belong to the casein kinase 1 superfamily of kinases. TTBK2 is ubiquitously expressed, while TTBK1 is neuron-specific and can directly phosphorylate tau proteins at multiple Ser/Thr residues that are found on paired helical filament-tau or PHF-tau in Alzheimer′s disease (AD) brains. Consistently, transgenic mice over-expressiong human TTBK1 develop age-dependent pathology in brain, including intraneuronal accumulation of phosphorylated neurofilament (p-NF), tau phosphorylation, cortical and hippocampal astro/microgliosis, calpain I up-regulation, enhanced CDK5/p35 complex formation, down regulation of NMDA receptor, and memory impairment.

Expected to react with both spliced isoforms reported by UniProt (Q5TCY1-1 and Q5TCY1-2). Clone F287-1.1-1E9 detected the full-length as well as the proteolytically processed human and mouse TTBK1 (Sato, S., et al. (Xu, J., et al. (2010). FASEB J. 24(8):2904-2915; 2008). J. Neurosci. 28(53):14511-14521).

Epitope: Kinase domain.

His-tagged recombinant fragment within the kinase domain of human TTBK1.

Detect TTBK1 using this Anti-TTBK1 Antibody, clone F287-1.1-1E9 validated for use in Western Blotting, Immunohistochemistry.

Immunohistochemistry Analysis: A representative lot detected a much higher TTBK1 immunoreactivity in paraformaldehyde-fixed frozen hippocampus and cortex sections from mice with human TTBK1 transgene (Xu, J., et al. (2010). FASEB J. 24(8):2904-2915).
Immunohistochemistry Analysis: A representative lot detected a much higher TTBK1 immunoreactivity in the subiculum and external pyramidal layer of visual cortex in paraformaldehyde-fixed frozen brain sections from transgenic mice overexpressing human TTBK1 (Sato, S., et al. (2008). J. Neurosci. 28(53):14511-14521).
Western Blotting Analysis: Representative lots detected both the full-length and the proteolytically processed murine TTBK1 in tissues (cerebellum, cortex, spinal cord) from wild-type mice, as well as the expression of human TTBK1 transgene in tissues from transgenic mice (Sato, S., et al. (Xu, J., et al. (2010). FASEB J. 24(8):2904-2915; 2008). J. Neurosci. 28(53):14511-14521).
Western Blotting Analysis: A representative lot detected an upregulation of both the full-length and the proteolytically processed TTBK1 in the frontal cortex gray matter extracts from Alzheimer′s diseased (AD) human brains than age-matched control non-AD brains (Sato, S., et al. (2008). J. Neurosci. 28(53):14511-14521).

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Evaluated by Western Blotting in TTBK1 trangenic mouse brain tissue lysate.

Western Blotting Analysis: 2.5 µg/mL of this antibody detected the expression of human TTBK1 transgene in 20 µg of transgenic mouse brain tissue lysate.

~142 observed. 142.7 kDa (isoform 1) and 69.08 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.