Anti-Vitamin D3 Receptor Antibody, clone 6F3.1

Vitamin D3 receptor (UniProt P11473; also known as 1,25-dihydroxyvitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1, VDR) is encoded by the VDR (also known as NR1I1) gene (Gene ID 7421) in human. VDR is a member of the superfamily of nuclear receptors for steroid hormones and acts as a ligand-inducible transcription factor that targets vitamin D responsive elements. Upon ligand binding, VDR complexes with the retinoid-X receptor (RXR), the heterodimer then targets hormone-response elements, resulting in transcription or transrepression of specific genes. Ligand binding also induces a conformational change at the C-terminal ligand-binding domain (LBD; a.a. 192-427), resulting in recruitment of the p160/SRC family coactivators whose acetylase activity plays an important role in transcription modulations via chromatin remodeling. VDR gene mutations are associated with type II vitamin D-resistant rickets and alopecia (hair loss).

Clone 6F3.1 targets an epitope present at the N-terminal half of both spliced isoforms of human vitamin D3 receptor reported by UniProt (P11473).

GST-tagged recombinant fragment corresponding to the N-terninal of human vitamin D3 receptor.

Anti-Vitamin D3 Receptor Antibody, clone 6F3.1, Cat. No. MABN1601, is a highly specific mouse monoclonal antibody that targets Vitamin D3 receptor and has been tested in Western Blotting.

Research Category
Neuroscience

Evaluated by Western Blotting in T47D cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected vitamin D3 receptor in 10 µg of T47D human breast cancer cell lysate.

~50 kDa observed. 48.29/53.88 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

Protein G purified.

Format: Purified

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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