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![Anti-phospho-TDP-43 [pSer409] antibody produced in rabbit ~1.0 mg/mL, affinity isolated antibody](/deepweb/assets/sigmaaldrich/product/images/407/478/bcd6d544-d187-42ed-8c1e-98bf709b4da7/640/bcd6d544-d187-42ed-8c1e-98bf709b4da7.jpg)
生物来源
rat
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
1D3, monoclonal
种属反应性
human
技术
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型
IgG2aκ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
phosphorylation (pSer409/pSer410)
基因信息
human ... TARDBP(23435)
一般描述
TAR DNA-binding protein 43 (TDP-43) belongs to the hnRNP protein family and plays an important role in transcription, pre-mRNA splicing, mRNA stability and mRNA transport. It is involved in splicing of the apolipoprotein A-II and cystic fibrosis transmembrane gene. This protein is highly expressed in the pancreas, placenta, lung, genital tract and spleen. Mutations in TDP-43 have been associated with amyotrophic lateral sclerosis, frontotemporal dementia, Parkinson′s disease and Alzheimer′s disease.
特异性
This antibody recognizes TDP-43 when phosphorylated at Ser409/Ser410.
免疫原
Epitope: Ser409/Ser410
Ovalbumin-conjugated linear peptide corresponding to human TDP-43 phosphorylated at Ser409/Ser410.
应用
Anti-phospho TDP-43 (Ser409/Ser410) Antibody, clone 1D3 is an antibody against phospho TDP-43 (Ser409/Ser410) for use in WB, ELISA, IH.
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in hippocampus from FTLD-U brain tissue. (Neumann, M., et al. (2009). Acta Neuropathol. 117:137–149.)
ELISA Analysis: A previous lot was used by an independent laboratory in ELISA. (Neumann, M., et al. (2009). Acta Neuropathol. 117:137–149.)
ELISA Analysis: A previous lot was used by an independent laboratory in ELISA. (Neumann, M., et al. (2009). Acta Neuropathol. 117:137–149.)
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
质量
Evaluated by Western Blot in human placenta cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected phosphorylated TDP-43 on 10 µg of human placenta cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected phosphorylated TDP-43 on 10 µg of human placenta cell lysate.
目标描述
50/25 kDa Observed
1) The 25-kDa C-terminal fragment is more prone to phosphorylation at Ser409/Ser410 than full-length TDP-43. (Zhang, Y.J., et al. (2009). PNAS. 106:7607-7612.)
2) 50-52 kDa observed MW . (Buratti, E., et al. (2001). The EMBO Journal 20:1774-1784.)
1) The 25-kDa C-terminal fragment is more prone to phosphorylation at Ser409/Ser410 than full-length TDP-43. (Zhang, Y.J., et al. (2009). PNAS. 106:7607-7612.)
2) 50-52 kDa observed MW . (Buratti, E., et al. (2001). The EMBO Journal 20:1774-1784.)
外形
Protein G Purified
Format: Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Human placenta cell lysate
Human placenta cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10-13 - German Storage Class 10 to 13
Patrick J Bosque et al.
PloS one, 8(5), e62301-e62301 (2013-05-25)
The transactive response DNA-binding protein (TDP-43) is a major component of the abnormal intracellular inclusions that occur in two common neurodegenerative diseases of humans: (1) a subtype of frontotemporal lobar degeneration and (2) amyotrophic lateral sclerosis. Genetics, experiments in cultured
Zhi Huang et al.
Nature communications, 14(1), 2747-2747 (2023-05-13)
Resilience to Alzheimer's disease is an uncommon combination of high disease burden without dementia that offers valuable insights into limiting clinical impact. Here we assessed 43 research participants meeting stringent criteria, 11 healthy controls, 12 resilience to Alzheimer's disease and
Pol Andrés-Benito et al.
Journal of neuropathology and experimental neurology, 78(5), 416-425 (2019-04-03)
Dyneins are major components of microtubules. Dynein assembly is modulated by a heterogeneous group of dynein axonemal assembly factors (DNAAFs). The present study analyzes dynein axonemal assembly factor 1 (DNAAF1) and leucine-rich repeat-containing protein 50 (LRRC50), the corresponding encoded protein
Cecilia S Lee et al.
Brain pathology (Zurich, Switzerland), 31(6), e12974-e12974 (2021-05-20)
People who have Alzheimer's disease neuropathologic change (ADNC) typically associated with dementia but not the associated cognitive decline can be considered to be "resilient" to the effects of ADNC. We have previously reported lower neocortical levels of hyperphosphorylated tau (pTau)
Isidro Ferrer et al.
Journal of neuropathology and experimental neurology, 80(3), 229-239 (2021-01-10)
Sporadic amyotrophic lateral sclerosis (sALS) and FTLD-TDP are neurodegenerative diseases within the spectrum of TDP-43 proteinopathies. Since abnormal blood vessels and altered blood-brain barrier have been described in sALS, we wanted to know whether TDP-43 pathology also occurs in blood
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