产品名称
Anti-Robo2 Antibody, clone 4C6.1, clone 4C6.1, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4C6.1, monoclonal
species reactivity
mouse, human, rat
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ROBO2(6092)
Analysis Note
Control
Mouse neuronal stem cell lysate
Mouse neuronal stem cell lysate
Evaluated by Western Blotting in mouse neuronal stem cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Robo2 in 200 µg of mouse neuronal stem cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Robo2 in 200 µg of mouse neuronal stem cell lysate.
Application
Detect Roundabout homolog 2 using this mouse monoclonal antibody, Anti-Robo2 Antibody, clone 4C6.1 validated for use in western blotting & IHC (Paraffin).
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Robo2 in rat brain (hypothalamus) and human brain tissue.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Signaling
Developmental Signaling
Biochem/physiol Actions
This antibody recognizes the cytoplasmic domain of Robo2
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Roundabout homolog 2 (Robo2) is a receptor for SLIT2, and likely SLIT1, which are thought to help guide cellular migration, including axonal navigation of the neural tube and the projection of axons during neuronal development. Vesicoureteral reflux type 2 (VUR2) has been attributed to defects in Robo2.
~ 170 kDa observed. The calculated molecular weight is 150 kDa, however Robo2 has been shown as a ~170 kDa band in western blots due to high glycosylation. (Fricke, C., et al. (2001). Science. 292:(5516):507-510).
Immunogen
Epitope: Cytoplasmic domain
GST-tagged recombinant protein corresponding to the cytoplasmic domain of human Robo2.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
未找到合适的产品？
试试我们的产品选型工具.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Anna Pisarek-Horowitz et al.
The American journal of pathology, 190(4), 799-816 (2020-03-30)
Roundabout guidance receptor 2 (ROBO2) plays an important role during early kidney development. ROBO2 is expressed in podocytes, inhibits nephrin-induced actin polymerization, down-regulates nonmuscle myosin IIA activity, and destabilizes kidney podocyte adhesion. However, the role of ROBO2 during kidney injury
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持