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生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
12D6.1, monoclonal
种属反应性
human
技术
immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型
IgG2aκ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... PRNP(5621)
一般描述
Major prion protein (UniProt P04156; also known as ASCR, CD230, PrP, PrP27-30, PrP33-35C) is encoded by the PRNP (also known as ALTPRP, CJD, GSD, HDL1, KURU, PRIP, PRP, SENF) gene (Gene ID 5621) in human. The major prion protein (PrP) is synthesized with an N-terminal signal peptide (a.a. 1-22) and a C-terminal proptptide (a.a. 231-253) sequence, which are posttranslationally removed to yield the mature (a.a. 23-230) N-glycosylated, glycosylphosphatidylinositol (GPI)-anchored cell surface protein. Structurally, PrP consists of a long, disordered, flexible NH2-proximal region and a globular COOH-proximal domain linked together by a hydrophobic core (HC) region (a,a. 111-134) that is considered as a key region in both physiological and disease related processes involving the prion protein. The N-terminal region is composed of five octapeptide (PHGGGWGQ) repeats (a.a. 51-91) sandwiched between two positively charged clusters, CC1 & CC2 (a.a. 23-27 & 95-110). The exact physiological function of PrP is not known, although various biological functions have been suggested for this protein, including signal transduction, neurotransmitter metabolism, cell adhesion, antioxidant activity, neurogenesis, immune cell activation, copper metabolism and homeostasis of trace elements. Transmissible spongiform encephalopathies (TSEs) represent a family of rare and fatal neurodegenerative disorders, including Creutzfeldt-Jakob disease, Gerstmann-Sträussler-Scheinker syndrome, fatal familial insomnia and kuru in human, bovine spongiform encephalopathy in cattle, and scrapie in sheep. It is widely accepted that the conformational transition of the native and predominantly α-helical cellular PrP (PrPC) to a β-sheet-rich pathogenic scrapie isoform (PrPSc) is responsible for the accumulation of PrPSc aggregates.
特异性
Clone 12D6.1 detects an epitope present in human PrP and the spliced isoform PrP(M8) reported by UniProt (P04156-1 & P04156-2), but absent in the alternative prion protein AltPrP (UniProt F7VJQ1-1).
免疫原
Epitope: Internal (N-terminal half).
Human IgG1 Fc-tagged recombinant human prion protein internal fragment.
应用
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected prion protein in human cerebral cortex tissue sections.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
This Anti-Prion Protein Antibody, clone 12D6.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Prion Protein.
质量
Evaluated by Western Blotting of recombinant human prion protein.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected 0.05 µg of human IgG1 Fc-tagged recombinant human prion protein internal fragment.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected 0.05 µg of human IgG1 Fc-tagged recombinant human prion protein internal fragment.
目标描述
27.66/26.89 kDa (PrP/PrP(M8) prepro-form), 22.75/25.24 kDa (mature/pro-form) calculated.
联系
Replaces: MAB1562
外形
Protein G purified.
Format: Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
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