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MABF81

Sigma-Aldrich

抗-ROR γT抗体,克隆6F3.1

clone 6F3.1, from mouse

别名:

Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, Nuclear receptor subfamily 1 group F member 3, Retinoid-related orphan receptor-gamma

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

6F3.1, monoclonal

种属反应性

human

技术

immunohistochemistry: suitable
western blot: suitable

同位素/亚型

IgG2aκ

NCBI登记号

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... RORC (6097)

一般描述

类视黄醇相关的孤儿受体-γ t(RORγT)是属于核激素受体家族的两种RORγ亚型之一,并包含单个核受体DNA结合结构域。RORγT是TNFDF6/FasL表达和IL2的T细胞产生的负调节剂。RORγT的上调是TH17细胞分化的要求。TGF-β’s与Foxp3的相互作用诱导了RORγT的抑制。主要在未成熟的胸腺细胞中观察到表达,并且在胎儿发育过程中,仅在淋巴诱导(LTi)细胞中发现表达。

免疫原

GST标记的重组蛋白,对应于人RORγt。

应用

免疫组织化学分析:该抗体以1:50稀释度在正常人肝组织中检测到RORγT。
研究子类别
炎症 & 自身免疫机制
研究类别
炎症 & 免疫学
经验证,该抗RORγT抗体(克隆6F3.1)可用于WB、IH中检测RORγT。

质量

通过蛋白质印迹法在T47D细胞裂解物中进行评估。

蛋白质印迹分析:1 µg/mL的该抗体在10 µg的T47D细胞裂解物中检测到RORγT。

目标描述

观测分子量〜55 kDa。在一些细胞裂解物中可观察到~55 kDa和~58 kDa的两种亚型。

外形

形式:纯化
纯化的小鼠单克隆IgG2aκ,溶于含0.1 M Tris-甘氨酸(pH 7.4)、150 mM NaCl和0.05%叠氮化钠的缓冲液中。
纯化蛋白G

储存及稳定性

自接收之日起,在2-8°C下可稳定保存1年。

分析说明

对照
T47D细胞裂解物

其他说明

浓度:关于批次特定浓度请参见检验报告。

免责声明

除非我们的目录或产品随附的其他公司文件中另有说明,否则我们的产品预期仅用于研究用途,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。

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WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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