Anti-Activation-induced cytidine deaminase (AID) Antibody, clone 328.8

Activation-induced cytidine deaminase (AID) is a 24 kDa, RNA-editing protein that is necessary for the initiation of both somatic hypermutation (SHM) and class switch recombination (CSR) of the Ig gene through the production of U:G mismatches in DNA, and is primarily found in activated B cells. AID expression has also been observed in several varying types of B cell lymphomas such as; Burkitt’s lymphoma, Hodgkin’s lymphoma, hairy cell lymphoma, follicular lymphoma, mantle cell lymphoma, mucosa-associated lymphoid tissue lymphoma, and mediastinal B cell lymphoma. Mutations in AID expression are causal to autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2), which is characterized by serum containing IgM but lacking IgG, IgA and IgE, which leads to severe susceptibility to bacterial infections.

KLH-conjugated linear peptide corresponding to human Activation-induced cytidine deaminase (AID).

Anti-Activation-induced cytidine deaminase (AID) Antibody, clone 328.8 detects level of Activation-induced cytidine deaminase (AID) & has been published & validated for use in WB, IH.

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH. (Matthew Scharff, Albert Einstein, matthew.scharff@einstein.yu.edu.)

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Evaluated by Western Blot in Raji cell lysate.

Western Blot Analysis: 1:2,000 dilution of this antibody detected Activation-induced cytidine deaminase (AID) on 10 µg of Raji cell lysate.

~ 24 kDa observed. Uncharacterized bands may be observed in some lysates.

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Raji cell lysate

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