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生物来源
rat
质量水平
偶联物
PE-Cy7
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
PC61.5, monoclonal
种属反应性
mouse
包装
antibody small pack of 25 μg
浓度
0.2 mg/mL
技术
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
同位素/亚型
IgG1λ
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
mouse ... Il2Ra(16184)
一般描述
The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, althoµgh with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells.The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naïve or conventional T cells which are CD25-.
免疫原
Immunogen information for this antibody is proprietary information.
应用
Research Category
Inflammation & Immunology
Inflammation & Immunology
This rat monoclonal antibody Anti-CD25 (mouse), PE-Cy7, clone PC61.5 validated for use in IP, IHC & Flow Cytometry for the detection of CD25.
质量
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.125 µg of this antibody detected CD25 in one million C57Bl/6 splenocytes.
Flow Cytometry Analysis: 0.125 µg of this antibody detected CD25 in one million C57Bl/6 splenocytes.
外形
Size Exclusion Chromatography
Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
储存及稳定性
It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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