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MABF33

Sigma-Aldrich

抗-MHC II类(I-A/I-E)抗体,克隆M5/114

clone M5/114, from rat

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别名:
CD74 molecule, major histocompatibility complex, class II invariant chain, Ia-associated invariant chain, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), gamma chain of class II antigens, Ia antigen-
UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

rat

质量水平

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

M5/114, monoclonal

种属反应性

mouse

技术

flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

同位素/亚型

IgG2bκ

NCBI登记号

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

mouse ... Cd74(16149)

一般描述

MHC II类相关不变量链(I-A/I-E),也称Ia抗原相关不变量链,是含有单个甲状腺球蛋白I型结构域的单通道II型膜糖蛋白。它们是MHC II类抗原处理过程中的关键组成部分,在α / β异二聚体合成后形成稳定复合物,并协调复合物从发生II类肽装载的ER区转移。

应用

使用经验证可用于WB、FC、IH的该抗-MHC II类(I-A/I-E)抗体,克隆M5/114检测MHC II类(I-A/I-E)。
流式细胞分析:先前批次已被独立实验室用于脾细胞FC。(Bhattacharya, A., et al. (1981). The Journal of immunology.127(6):2488-2496。)

免疫组织化学分析:先前批次已被独立实验室用于胸腺组织IH。(de Bont, E., et al. (1999). Int. Immunol. 11(8):1295-1306.)

质量

已通过蛋白质印迹在小鼠脾组织裂解液中进行了评估。

蛋白质印迹分析:1 µg/mL该抗体在10 µg小鼠脾组织裂解液中检测到MHC II类(I-A/I-E)。

目标描述

观测值〜32 kDa。
选择性剪接产生2种亚型:32 kDa(长亚型)和 24 kDa(短亚型)。

外形

形式:纯化

其他说明

浓度:请参考批次特异性浓缩物的检验报告。

WGK

WGK 1


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