Anti-M-CSF Antibody, clone 3G6.4.8

Macrophage colony-stimulating factor 1 (UniProt: P09603; also known as CSF-1, M-CSF, MCSF, Lanimostim) is encoded by the CSF1 gene (Gene ID: 1435) in human. M-CSF is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-32), which is subsequently cleaved off to generate the mature form that contains a lumenal domain (aa 33-496), a transmembrane domain (aa 497-517), and a cytoplasmic domain (aa 518-554). It can be present as a disulfide-linked homodimer or heterodimer. Two distinct biologically active forms of M-CSF have been described: a membrane-bound and a secreted form that result from alternative splicing of the CSF1 gene. It plays an essential role in the regulation of survival, proliferation, and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. It promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. M-CSF is also involved in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and for normal bone development. Its biological effects are mediated via binding to CSF-1R/c-Fms receptor that is expressed by multipotent hematopoietic cells, mononuclear phagocyte progenitor cells, monocytes, tissue macrophages, and osteoclasts. Aberrant expression of M-CSF or CSF-1R is reported to promote cancer cell proliferation, invasion and formation of metastases. Overexpression of M-CSF or CSF-1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers.

Clone 3G6.4.8 is a mouse monoclonal antibody that detects human macrophage colony-stimulating factor 1 (M-CSF). It targets an epitope within the N-terminal half.

A recombinant fragment corresponding to 149 amino acids from the N-terminal half of human Macrophage colony-stimulating factor 1 (M-CSF) with a GHERQSEGS sequence attached at its C-terminal end.

Quality Control Testing

Evaluated by Western Blotting with recombinant Human M-CSF.

Western Blotting Analysis (WB): A 1:100,000 dilution of this antibody detected recombinant human M-CSF under non-denaturing conditions.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected M-CSF in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-M-CSF, clone 3G6.4.8, Cat. No. MABF3177, is a mouse monoclonal antibody that detects M-CSF and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.