Anti-SARS CoV-2 RBD mutant N501Y Antibody, clone 8A6

Spike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. Over the course of the pandemic, SARS-CoV2 has consistently mutated giving rise to various mutants. Clone 8A6 recognizes SARS-CoV-2 spike protein with N501Y mutation. Several variants, including B.2.2.7, B.1.351, and P.1 share this mutation on the receptor binding domain (RBD) and is a more infectious variant. RBD with N501Y mutation exhibits a stronger interaction with ACE2 receptor and slower dissociation rate. (Ref.: Liu, Y., et al. (2022). Nature. 602(7696); 294-299; Tian, F., et al. (2021). eLife. 10; e69091).

KLH-conjugated linear peptide segment surrounding N501Y mutant site.

Quality Control Testing

Evaluated by Western Blotting with recombinant SARS-CoV-2 Spike RBD (N501Y) protein .

Western Blotting Analysis: A 1:500 dilution of this antibody detected recombinant SARS-CoV-2 RBD mutant N501Y protein.

Tested Applications

ELISA Analysis: Serial dilutions of this antibody detected various recombinant SARS-CoV-2 protein samples

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 8A6 is a mouse monoclonal antibody that detects N501Y mutant form of of SARS-CoV-2 spike protein.

Purified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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