Anti-SARS-CoV-2 nucleocapsid NC1 Antibody, clone 6G3-G6

Nucleoprotein (UniProt: P0DTC9; also known as Nucleocapsid protein, NC, Protein N) is encoded by the N gene (Gene ID: 1489678) in Human SARS-2 coronavirus. The SARS-CoV-2 (COVID-19 causing virus) is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). Nucleoprotein of SARS-CoV-2 is an homooligomeric protein that packages the positive strand viral genome RNA into helical ribonucleocapsid and plays a role in virion assembly through its interactions with the viral genome and membrane protein M. It plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Nucleocapsid protein can be divided into five domains: an intrinsically disordered N-terminal domain (NTD), an RNA-binding domain (RBD), a predicted disordered central linker (LINK), a dimerization domain, and a disordered C-terminal domain. The NTD is thought to mediate a specific interaction with the viral genome s packaging signal, and the CTD forms a compact dimer that has been proposed to aid vRNP assembly. The RNA binding region of SARS-CoV-2 is localized to amino acids 41-186 and the dimerization domain resides in amino acids 258-361. The complete N protein of SARS- COV-2 is a highly basic with positive net charges and high pI (10.11). Its N-terminal region is more basic with positive charge, while the C-terminal region is acidic with negative charge. The middle region of nucleoprotein displays relatively higher hydrophobicity and the two termini display greater hydrophilicity. Nucleoprotein can undergo phosphorylation at multiple serines by GSK-3, CKII, Akt, CDKs, and Polo-like kinases. Some of these phosphorylation events are essential for RNA binding, oligomerization and localization to nucleoli. Phosphorylation is also required for recruitment of host RNA helicase DDX1 (DEAD-Box Helicase 1) that facilitates template readthrough and enables longer subgenomic mRNA synthesis. (Ref.: Cubuk, J., et al. (2021). Nat. Commun. 12; Article number: 1936; Lu, S., et al. (2021). Nat. Commun . 12; Article number 502; Miranda, J., et al. (2021). Mol. Med. 27; Article 161).

Clone 6G3-G6 is a mouse monoclonal antibody that detects Nucleocapsid protein of SARS-CoV-2. It targets an epitope within the NC1 region.

His-tagged full-length recombinant Nucleocapsid protein of SARS-CoV-2.

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293T cells expressing SARS-CoV-2-Nucleocapsid NC1 protein.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Nucleoprotein NC1 in lysate from HEK293T cells expressing SARS-CoV-2 nucleocapsid NC1, but not in lysates from wild-type HEK293T cells or from HEK293T cells expressing the Spike protein.

Tested Applications

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected Nucleoprotein in Whole cell lysates of HEK293T expressing the nucleocapsid (NC) proteins of the indicated human coronaviruses (Data courtesy of Dr. Stefan Schüchner, Dr. Egon Ogris, Max Perutz Labs, Medical University of Vienna, Austria).

Immunocytochemistry Analysis: A representative lot detected Nucleoprotein in HeLa cells transiently transfected with SARS-CoV-2 NC-P2A-GFP (Data courtesy of Dr. Stefan Schüchner, Dr. Egon Ogris, Max Perutz Labs, Medical University of Vienna, Austria).

Immunoprecipitation Analysis: A representative lot detected Nucleoprotein in with the indicated monoclonal antibodies from whole cell lysate of HEK293T cells expressing Sars-CoV-2-NC-P2A GFP (Data courtesy of Dr. Stefan Schüchner, Dr. Egon Ogris, Max Perutz Labs, Medical University of Vienna, Austria).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SARS-CoV-2 nucleocapsid NC1 fragment, clone 6G3-G6, Cat. No. MABF2817, is a mouse monoclonal antibody that detects Nucleoprotein and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.