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安全信息

MABF2812

Sigma-Aldrich

Anti-CD137 Antibody, clone LOB12.0

别名:

4-1BB ligand receptor, T-cell antigen 4-1BB, Tumor necrosis factor receptor superfamily member 9

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

rat

质量水平

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

LOB12.0, monoclonal

分子量

calculated mol wt 27.6 kDa
observed mol wt ~N/A kDa

纯化方式

using protein G

种属反应性

mouse

包装

antibody small pack of 100

技术

competitive inhibition ELISA: suitable
flow cytometry: suitable
inhibition assay: suitable
surface plasmon resonance (SPR): suitable

同位素/亚型

IgG2aκ

表位序列

Unknown

Protein ID登记号

UniProt登记号

储存温度

-10 to -25°C

基因信息

mouse ... Tnfrsf9(21942)

特异性

Clone LOB12.0 is a rat monoclonal antibody that detects CD137 (Tnfrsf9).

免疫原

Mouse CD137 human Fc fusion protein

应用

Quality Control Testing

Isotype testing: Identity confirmation by Isotyping test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be rat IgG2a .

Tested Applications

Surface plasmon resonance: A representative lot detected CD137 in Surface plasmon resonance applications (Buchan, S.L., et al. (2018). Immunity. 49(5):958-970.e.7).

Flow Cytometry Analysis: 10 µg from a representative lot detected CD137 in Jurkat cells expressing mouse CD137.

Agonist Activity: A representative lot of this antibody co-stimulated CD8+ T cells and potentiated anti-tumor immunity in mice.(Taraban, V.Y., et al. (2002). Eur J Immunol. 32(12); 3617-3627 ).

Competition Assay: A representative lot of this antibody competed for binding to 4-1BB on effector T cells. (Buchan, S.L., et al. (2018). Immunity. 49(5):958-970.e.7).

Flow Cytometry Analysis: A representative lot detected CD137 in Flow Cytometry applications (Buchan, S.L., et al. (2018). Immunity. 49(5):958-970.e.7).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

目标描述

Tumor necrosis factor receptor superfamily member 9 (UniProt: P20334; also known as 4-1BB ligand receptor, T-cell antigen 4-1BB, CD137) is encoded by the Tnfrsf9 (also known as Cd137, IIa, Ly63) gene (Gene ID: 21942) in murine species. CD137 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 24-187), a transmembrane domain (aa 187-208), and a cytoplasmic domain (aa 209-256). It is a member of the TNFR superfamily that serves as an activation-induced costimulatory molecule. Its expression is observed on activated T cells, NK cells, dendritic cells, eosinophils, mast cells, endothelial cells, and some tumor cells. It is rapidly expressed on activated CD8+ T cells and at lower levels on activated CD4+ T cells. Induction of CD137 expression on CD8+ T cells is reported to be highly sensitive to low levels of TCR stimulation. Engagement of CD137 on T cells stimulates their proliferation independently of CD28 and promotes IL-2 and IFN- secretion by Th effector cells and CTLs, respectively. Expression of CD137 is shown to negatively correlate with lymphocyte proliferation and positively with the degree of activation-induced cell death caused by mitogen overstimulation. Treg cells that infiltrate tumors are reported to express higher levels of CD137 and administration of clone LOB12.0 with agonistic function promotes T cell agonism to promote tumor rejection. (Ref.: Buchan, SL., et al. (2018). Immunity 49(5), 958-970; Taraban, VY., et al. (2002). Eur. J. Immunol. 32(12); 3617-3627).

外形

Purified rat monoclonal antibody IgG2a in PBS without preservatives.

重悬

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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