Anti-TOX Antibody, clone NAN448B

Thymocyte selection-associated high mobility group box protein TOX (UniProt: O94900; also known as Thymus high mobility group box protein TOX) is encoded by the TOX (also known as KIAA0808) gene (Gene ID: 9760) in human. TOX is a member of an evolutionarily conserved DNA-binding protein family that plays multiple roles in the development of the adaptive immune system. It acts as a developmental checkpoint and regulates thymocyte positive selection toward T cell lineage commitment. It is required for the development of various T cell subsets, including CD4+ helper T-cells, CD8+ cytotoxic T-cells, regulatory T-cells, and CD1D-dependent natural killer T (NKT) cells. Its nuclear localization signal lies in amino acids 237-256 and the DNA-binding region (HMG box) resides in amino acids 161-329. TOX serves as a DNA-binding factor that regulates transcription by modifying local chromatin structure and modulating the formation of multi-protein complexes. It binds to GC-rich DNA sequences in the proximity of transcription start sites to alter chromatin structure and modify access of transcription factors to DNA. It also plays a major role in neural stem cell commitment and corticogenesis. During cortical development, it controls the neural stem cell pool by inhibiting the switch from proliferative to differentiating progenitors. It also promotes neurite outgrowth in newborn neurons migrating to reach the cortical plate. In T-cell lymphomas high TOX expression has been described in all the Mycosis fungoides (MF). A majority of precursor B/T lymphoblastic, follicular and diffuse large B-cell lymphomas, nodular lymphocyte-predominant Hodgkin lymphomas and angioimmunoblastic T-cell lymphomas strongly expressed the TOX protein. (Ref.: Maestre, L., et al. (2020). PLoS ONE 15(2); e0229743; Yun, S., et al. (2011). Immunol. Lett. 136(1); 29-36).

Clone NAN448B is a rat monoclonal antibody that detects human Thymocyte selection-associated high mobility group box protein TOX. It targets an epitope within the N-terminal half

His-tagged recombinant fragment corresponding to the first 250 amino acids form human Thymocyte selection-associated high mobility group box protein TOX (TOX).

Quality Control Testing

Evaluated by Western Blotting in MOLT-4 cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TOX in MOLT-4 cell lysates.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected TOX in Immunocytochemistry applications (Maestre, L., et al. (2020). PLoS One. 15(2):e0229743).

Immunohistochemistry Applications: A representative lot detected TOX in Immunohistochemistry applications (Maestre, L., et al. (2020). PLoS One. 15(2):e0229743).

Immunofluorescence Analysis: A representative lot detected TOX in Immunofluorescence applications (Maestre, L., et al. (2020). PLoS One. 15(2):e0229743).

Western Blotting Analysis: A representative lot detected TOX in Western Blotting applications (Maestre, L., et al. (2020). PLoS One. 15(2):e0229743).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-TOX, clone NAN448B, Cat. No. MABF2794, is a rat monoclonal antibody that detects Thymus high mobility group box protein TOX and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.