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生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
11–4.1, monoclonal
分子量
calculated mol wt 22.33 kDa
observed mol wt ~19 kDa
种属反应性
human
包装
antibody small pack of 100 μL
技术
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG
运输
dry ice
储存温度
-10 to -25°C
靶向翻译后修饰
unmodified
特异性
Clone 11-4.1 is a mouse monoclonal antibody that detects human IL-18 protein. It targets an epitope within 6 amino acids from the N-terminal half.
免疫原
His-tagged full-length active recombinant human Interleukin-18 (without the propeptide).
应用
Quality Control TestingEvaluated by Western Blotting in HeLa cell lysate.Western Blotting Analysis: A 1:500 dilution of this antibody detected IL-18 in HeLa cell lysate.Tested ApplicationsImmunoprecipitation Analysis: A representative lot immunoprecipitated IL-18 in Immunoprecipitation applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).Immunocytochemistry Analysis: A representative lot detected IL-18 in Immunocytochemistry applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).Western Blotting Analysis: A representative lot detected IL-18 in Western Blotting applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected IL-18 in HeLa cells.Inhibition Assay: A representative lot inhibited IL-18 involved interferon release from KG-1 cells.(Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82). Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-IL-18, clone 11 4.1, Cat. No. MABF2674, is a mouse monoclonal antibody that detects Interleukin-18 and is tested for use in Immunocytochemistry, Immunoprecipitation, Inhibition Assay, and Western Blotting.
目标描述
Interleukin-18 (UniProt: Q14116; also known as IL-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma) is encoded by the IL18 (also known as IGIF, IL1F4) gene (Gene ID: 3606) in human. IL-18 is a pro-inflammatory cytokine that is shown to evoke both innate and acquired responses. It is synthesized with a propeptide (aa 1-36), which is subsequently cleaved off by caspases 1 and 4 to generate the active mature form. IL-18 is reported to be constitutively expressed in monocytes, macrophages, epithelial cells, osteoblasts, keratinocytes, and synovial fibroblasts. Its expression has also been reported in adrenal cortical cells. Mature IL-18 acts by binding to the IL-18 receptor a (IL-18a) to form a low affinity binary complex that recruits IL-18b to form a high affinity complex that leads to the activation of NF-kB and MAP kinase pathways. IL-18 can synergize with IL-12 to induce interferon synthesis from T-helper 1 and natural killer cells. Clone 11-4.1 is shown to block IL-18 evolved interferon release in a dose-dependent manner (IC50 = 14.2 nM). (Ref.: Nariai, Y., et al. (2019). Arch. Biochem. Biophys. 663; 71-82).
外形
Purified mouse monoclonal antibody IgG2b in PBS without preservatives.
储存及稳定性
Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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