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Merck
CN

MABF2316

Anti-MCPyV T-antigen Antibody, clone 2t2

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
2t2, monoclonal
Species reactivity:
virus, mouse
Application:
ICC, IHC, WB
Citations:
-
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antibody form

purified antibody

clone

2t2, monoclonal

purified by

using protein G

species reactivity

virus, mouse

concentration

(Please refer to lot specific datasheet.)

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG1κ

UniProt accession no.

General description

A linear peptide corresponding to 17 amino acids from the J-domain region present in both MCV LT and sT proteins.
~17 and 48 kDa observed; 28.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Application

Immunohistochemistry Analysis: A representative lot detected MCPyV T-antigen in Immunohistochemistry applications (Toptan, T., et. al. (2016). JCI Insight. 1(2)).

Western Blotting Analysis: A representative lot detected MCPyV T-antigen in Western Blotting applications (Wang, X., et. al. (2012). PLoS Pathog. 8(11); Shuda, M., et. al. (2014). J Invest Dermatol. 134(5):1479-1481; Toptan, T., et. al. (2016). JCI Insight. 1(2)).

Immunocytochemistry Analysis: A representative lot detected MCPyV T-antigen in Immunocytochemistry applications (Toptan, T., et. al. (2016). JCI Insight. 1(2)).

Biochem/physiol Actions

Clone 2t2 is a mouse monoclonal antibody that detects MCV LT and the 57 kDa T.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in MKL-1 cell lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected MCPyV T-antigen in MKL-1 cell lysates.

Other Notes

Virus transformed human cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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