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抗体形式
purified antibody
克隆
2t2
monoclonal
纯化方式
using protein G
种属反应性
virus, mouse
浓度
(Please refer to lot specific datasheet.)
技术
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型
IgG1κ
UniProt登记号
一般描述
A linear peptide corresponding to 17 amino acids from the J-domain region present in both MCV LT and sT proteins.
特异性
Clone 2t2 is a mouse monoclonal antibody that detects MCV LT and the 57 kDa T.
应用
Immunohistochemistry Analysis: A representative lot detected MCPyV T-antigen in Immunohistochemistry applications (Toptan, T., et. al. (2016). JCI Insight. 1(2)).
Western Blotting Analysis: A representative lot detected MCPyV T-antigen in Western Blotting applications (Wang, X., et. al. (2012). PLoS Pathog. 8(11); Shuda, M., et. al. (2014). J Invest Dermatol. 134(5):1479-1481; Toptan, T., et. al. (2016). JCI Insight. 1(2)).
Immunocytochemistry Analysis: A representative lot detected MCPyV T-antigen in Immunocytochemistry applications (Toptan, T., et. al. (2016). JCI Insight. 1(2)).
Western Blotting Analysis: A representative lot detected MCPyV T-antigen in Western Blotting applications (Wang, X., et. al. (2012). PLoS Pathog. 8(11); Shuda, M., et. al. (2014). J Invest Dermatol. 134(5):1479-1481; Toptan, T., et. al. (2016). JCI Insight. 1(2)).
Immunocytochemistry Analysis: A representative lot detected MCPyV T-antigen in Immunocytochemistry applications (Toptan, T., et. al. (2016). JCI Insight. 1(2)).
质量
Evaluated by Western Blotting in MKL-1 cell lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected MCPyV T-antigen in MKL-1 cell lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected MCPyV T-antigen in MKL-1 cell lysates.
物理属性
~17 and 48 kDa observed; 28.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
外形
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Virus transformed human cells.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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