Anti-NKp30 (CD337) Antibody

Natural cytotoxicity triggering receptor 3 (UniProt: O14931; also known as Activating natural killer receptor p30, Natural killer cell p30-related protein, NK-p30, NKp30, CD337) is encoded by the NCR3 (also known as 1C7, LY117) gene (Gene ID: 259197) in human. NKp30 is a single-pass type I membrane protein that is selectively expressed by all resting and activated NK cells. Its weaker expression is observed in spleen. It serves as a receptor of natural killer/NK cell. It is activated by binding of extracellular ligands including BAG6 and NCR3LG1. It stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It cooperates with NKp46 and/or NKp44 in inducing NK-mediated cytotoxicity against the majority of target cells. It is also reported to control NK cells cytotoxicity against tumor cells. Its interaction with BAG6 promotes myeloid dendritic cells (DC) maturation, both through killing DCs that fail to achieve a mature phenotype and by inducing the release of TNF-alpha and interferon-gamma by NK cells that promote DC maturation. NKp30 is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 19-135), a transmembrane domain (aa 136-156), and a cytoplasmic domain (aa 157-201). Six isoforms of NKp30 have been described that are produced by alternative splicing.

Clone 1C01 is a mouse monoclonal antibody that detects human Natural cytotoxicity triggering receptor 3 (NKp30).

YT cells

Quality Control Testing

Evaluated by Western Blotting in Human spleen tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NKp30 (CD337) in Human spleen tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected NKp30 (CD337) in Human tonsil tissue lysate.

Function Analysis: A representative lot of this antibody blocked the binding of NKp30-Fc to β-1,3-Glucan and reduced Nkp30 clustering. (Li, S.S., et. al. (2018). Nat Commun. 9(1):751; Li, S.S., et. al. (2013). Cell Host Microbe. 14(4):387-97).

Western Blotting Analysis: A representative lot detected NKp30 (CD337) in Western Blotting applications (Li, S.S., et. al. (2018). Nat Commun. 9(1):751; Li, S.S., et. al. (2013). Cell Host Microbe. 14(4):387-97).

Immunoprecipitation Analysis: A representative lot immunoprecipitated NKp30 (CD337) in Immunoprecipitation applications ( Li, S.S., et. al. (2013). Cell Host Microbe. 14(4):387-97 ).

Flow Cytometry Analysis: A representative lot detected NKp30 (CD337) in Flow Cytometry applications (Li, S.S., et. al. (2018). Nat Commun. 9(1):751; Li, S.S., et. al. (2013). Cell Host Microbe. 14(4):387-97).

Immunocytochemistry Analysis: A representative lot detected NKp30 (CD337) in Immunocytochemitry applications (Li, S.S., et. al. (2013). Cell Host Microbe. 14(4):387-97).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-NKp30 (CD337), clone 1C01, Cat. No. MABF2237, is a This mouse monoclonal antibody that detects NKp30 (Cd337) and is tested for use in Western Blotting, Flow Cytometry, Function Analysis, Immunocytochemistry, and Inhibits Acitivty/Function.

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.