Anti-canine NKp46 (CD335) Antibody, clone 48A

Natural cytotoxicity triggering receptor 1 (UniProt: S4S7F8; also known as Lymphocyte antigen 94 homolog, NK cell-activating receptor, Natural killer cell p46-related protein, NK-p46, NKp46, CD335) is encoded by the NCR1 gene (Gene ID: 611390) in canine species. NK-p46 is a single-pass type I membrane protein that belongs to the natural cytotoxicity receptor (NCR) family and serves as a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis and elimination of virus-infected cells. It is considered as a primary activating receptor mediating NK cell antiviral function. It is involved in the functional recognition of viral HA and hemagglutinin-neuraminidases. Following the initiation of an NK-target cell interaction, NKp46 is reported to cluster at the cell membrane, specifically at the immune synapse where it mediates cytoskeletal rearrangement and cellular polarization. Canine NK-p46 is synthesized with a signal peptide of 20 amino acids, which is subsequently cleaved off. The mature form has an extracellular domain (aa 21-255), a helical domain (aa 256-279), and a cytoplasmic domain (aa 280-316). It also has an Ig domain (129-211). Clone 48A is shown to recognize CD3 /NKp46+ cells that display phenotypic and functional similarities to human NK cells. Also, it recognizes a subset of T cells that are NKp46 positive. (Ref.: Foltz, JA et al. (2016). Front. Immunol. 7; 521).

Clone 48A is a mouse monoclonal antibody that specifically detects canine NKp46.

L-cells expressing canine NKp46 fusion protein

Anti-canine NKp46 (CD335), clone 48A, Cat. No. MABF2109, is a mouse monoclonal antibody that detects canine Natural cytotoxicity triggering receptor 1(NKp46) and has been tested for use in ELISA, Flow Cytometry, and Immunocytochemistry.

ELISA Analysis: A representative lot detected canine NKp46 (CD335) in ELISA applications (Foltz, J.A., et. al. (2016). Front Immunol. 7:521).

Flow Cytometry Analysis: A representative lot detected canine NKp46 (CD335) in Flow Cytometry applications (Foltz, J.A., et. al. (2016). Front Immunol. 7:521).

Evaluated by Immunocytochemistry in canine peripheral blood mononuclear cells (PBMCs).

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected canine NKp46 (CD335) in canine peripheral blood mononuclear cells (PBMCs).

36.13 kDa calculated.

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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