Anti-Siglec-H Antibody, clone 551

Siglec-H (UniProt: Q3Y597; also known as Sialic acid binding Ig-like lectin H) is encoded by the Siglech gene (Gene ID: 233274) in murine species. Siglecs are sialic acid-binding immunoglobulin-like lectins that are expressed mostly in the immune system and may be involved in both adhesive and signaling functions. Siglec-H is a type I, CD33-related siglec-like transmembrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off in the mature form. The mature form has to ig-like domains (aa 32-141 and 151-234) in its extracellular region. Unlike other CD33-related siglecs, it lacks tyrosine-based signaling motifs in its cytoplasmic tail. Siglec-H is shown to be expressed on plasmaocytoid dendritic cells (pDCs) and can serve as a reliable marker for these cells. Siglec-H is also reported to serve as an endocytic receptor expressed on murine pDCs. Action of Siglec-H depends on a 12 kDa DNAX-activating protein (DAP). (Ref.: Zhang, J., et al. (2006). Blood 107(9); 3600-3608; Yun, TJ., et al., (2016), Cell Metabolism 23(5); 852-866).

Clone 551 is a rat monoclonal antibody that detects murine Siglec-H. It targets an epitope with in the extracellular domain.

Bone marrow-derived Type 1 interferon-producing cells.

Epitope: extracellular domain

Anti-Siglec-H, clone 551, Cat. No. MABF2045, is a rat monoclonal antibody that detects Sialic acid binding Ig-like lectin H and has been tested for use in Flow Cytometry and Immunohistochemistry.

Immunohistochemistry Analysis: A representative lot detected Siglec-H in Immunohistochemistry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).

Flow Cytometry Analysis: A representative lot detected Siglec-H in Flow Cytometry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).

Research Category
Inflammation & Immunology

Evaluated by Immunohistochemistry in frozen mouse spleen tissue sections.

Immunohistochemistry Analysis: A 1:25 dilution of this antibody detected Siglec-H in frozen mouse spleen tissue sections.

34.35 kDa calculated.

Protein G purified

Format: Purified

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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