Anti-IL-17A/CTLA8 Antibody, clone MM17F3

Interleukin-17A (UniProt: Q62386; also known as IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8) is encoded by the Il17A (also known as Ctla8, Il17) gene (Gene ID: 16171) in murine species. IL-17A is a homodimeric, pro-inflammatory cytokine that is synthesized with a signal peptide (aa 1-25) that is subsequently cleaved off to produce the mature protein. It is expressed by Th17 cell lineage, and its expression is observed in innate lymphoid cells, T cell subsets, and invariant natural killer (iNKT) cells. It is induced upon differentiation of CD4+ T cells toward Th17 effector cells upon antigen receptor binding in the presence of IL6 and TGF-β1. Its levels are also up-regulated by pro-inflammatory cytokines in response to microbes in various immune cells. IL-17A actions are mediated via interaction with IL-17RA and IL-17RC heterodimeric receptor complex, triggering homotypic interaction of these receptors with TRAF3 Interacting Protein 2 (TRAF3IP2) adapter. This leads to downstream TRAF6-mediated activation of NF-kB and MAP kinase pathways resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides, and matrix metalloproteinases. IL-17A plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. It plays important roles in multiple biological processes, including the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF- kB and co-stimulation of T cell proliferation. (Ref.: Lemaire, MM., et al. (2011). J. Immunol. 187(7); 3530 3537; Re, SL., et al. (2010). J. Immunol. 184(11); 6367-6377; Liang, SC., et al. (2007). J. Immunol. 179(11); 7791-7799; Park, H., et al. (2005). Nat. Immunol. 6(11); 1133-1141).

Clone MM17F3 is a mouse monoclonal antibody that detects IL-17A/CTLA8.

Ovalbumin-conjugated recombinant murine IL-17A.

Quality Control Testing

Evaluated by Western Blotting with recombinant mouse IL-17A.

Western Blotting Analysis (WB): 1:20,000 dilution of this antibody detected recombinant mouse IL-17A.

Tested Applications

Neutralizing: A representative lot neutralized the activity of Interleukin-17A in Neutralizing application (Galicia, G., et al. (2009). J Clin Immunol.;29(4):426-33; Kelchtermans, H., et al. (2009). Arthritis Res Ther.;11(4):R122).

Flow Cytometry Analysis: A representative lot detected Interleukin-17A in Flow Cytometry application (Lo Re, S., et al. (2010). J Immunol. 184(11):6367-77; Chen, X., et al. (2010). Biol Blood Marrow Transplant. 16(1):123-8).

ELISA Analysis: A representative lot detected Interleukin-17A in ELISA application (Uyttenhove, C., et al. (2006). Eur J Immunol. 36(11):2868-74; Lemaire, M.M., et al. (2011). J Immunol. 187(7):3530-7).

Immunohistochemistry Applications: A representative lot detected IL-17A/CTLA8 in Immunohistochemistry application (Chen, X., et al. (2010). Biol Blood Marrow Transplant. 16(1):123-8).

Inhibition Assay: A representative lot inhibited IL-6 induction by IL-17A in C11D fibroblastic cell line. (Uyttenhove, C., et al. (2006). Eur J Immunol.;36(11):2868-74)..

ELISA Analysis: Various dilutions of this antibody detected recombinant mouse IL-17A protein.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.