Anti-UBR5 Antibody, clone 16G10.1

E3 ubiquitin-protein ligase UBR5 (UniProt; O95071; also known as EC: 2.3.2.26, E3 ubiquitin-protein ligase, HECT domain-containing 1, HECT-type E3 ubiquitin transferase UBR5, Hyperplastic discs protein homolog, hHYD, Progestin-induced protein) is encoded by the UBR5 (also known as EDD, EDD1, HYD, KIAA0896) gene (Gene ID: 51366) in human. UBR5 is a widely expressed nuclear E3 ligase that is most abundant in testis and expressed at high levels in brain, pituitary and kidney. It is a component of the N-end rule pathway that recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. UBR5 is involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination and may play a role in control of cell cycle progression. It is shown to regulate DNA topoisomerase II binding protein (TopBP1) in the DNA damage response and may have a tumor suppressive function. It acts as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of Lys-63-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes. URB5 contains a zinc-finger domain (aa 1177-1245), a poly(A)-binding protein C-terminal (PABC) domain(aa 2377-2454), and a (Homologous to the E6-AP Carboxyl Terminus (HECT) domain (aa 2462-2799). Two isoforms of UBR5 have been described that are produced by alternative splicing.

Clone 16G10.1 detects E3 ubiquitin-protein ligage UBR5 in human cells. It targets an epitope within 181 amino acids from the C-terminal half.

GST/His-tagged recombinant fragment corresponding to 181 amino acids from the C-terminal half of human E3 ubiquitin-protein ligase UBR5.

Anti-UBR5, clone 16G10.1, Cat. No. MABF1110, is a mouse monoclonal antibody that detects E3 ubiquitin-protein ligase UBR5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected UBR5 in human cerebral cortex, human tonsil, and human prostate cancer tissues.

Research Category
Inflammation & Immunology

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected UBR5 in 10 µg of Raji cell lysate.

~305 kDa oserved; 309.35 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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