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此商品 | SAB4200617 | SAB4501939 | SAB4501938 |
|---|---|---|---|
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| Gene Information human ... MYBL2(4605) | Gene Information human ... MYB(4602) | Gene Information human ... MYBL2(4605) | Gene Information human ... MYBL1(4603) |
| species reactivity mouse, human | species reactivity human, mouse | species reactivity mouse, human, rat | species reactivity human, mouse |
| biological source mouse | biological source mouse | biological source rabbit | biological source rabbit |
| technique(s) western blot: suitable | technique(s) flow cytometry: 2.5-5 μg/test using using HL-60 cells., immunoblotting: 0.5-1 μg/mL using using Jurkat total cell extract., immunofluorescence: 2-4 μg/mL using using HeLa cells. | technique(s) ELISA: 1:20000, immunofluorescence: 1:100-1:500, western blot: 1:500-1:1000 | technique(s) ELISA: 1:10000, western blot: 1:500-1:1000 |
一般描述
B-Myb, also known as B-Myb or Myb-like protein 2, and encoded by the gene MYBL2/BMYB, is a transcription factor protein involved in the regulation of cell survival, proliferation and differentiation. B-Myb is phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. B-Myb is a component of the DREAM complex (also named LINC complex) that is composed of at least E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2 proteins. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL22. B-Myb is localized to the nucleus. B-Myb is widely expressed and up regulated in cancer cells. B-Myb is an oncogenic transcription factor involved in G2/M progression and is a key target of anti-cancer therapy.
免疫原
Recombinant protein corresponding to human B-Myb.
应用
Detect B-Myb using this Anti-B-Myb Antibody, clone LX015.1 validated for use in western blotting.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Western Blotting Analysis: A representative lot from an independent laboratory detected U2OS cell lysates expressing B-Myb in U2OS (Chen, X., et al. (2013). Mol Cel Biol. 33(2):227-236.).
Western Blotting Analysis: A representative lot from an independent laboratory in NIH/3T3 cell lysates and in undifferentiated and differentiated F9 cell lysates (Knight, A. S., et al. (2009). Oncogene. 28(15):1737-1747.).
Western Blotting Analysis: A representative lot from an independent laboratory in cycling NIH/3T3 and F9 nuclear extracts (Muller, G. A., et al. (2012). Nucleic Acids Res. 40(4):1561-78.).
Western Blotting Analysis: A representative lot from an independent laboratory detected B-Myb wild-type cycling MEF cell lysates and not in B-Myb wild type G0 phase arrested MEF cell lysates. The same lot also detected B-Myb in G0 phase arrested and in cycling MEF cell lysates inappropriately expressing homozygous mutant B-Myb (Tavner, F., et al. (2007). Oncogene. 26(19): 2727-2735.).
Western Blotting Analysis: A representative lot from an independent laboratory in NIH/3T3 cell lysates and in undifferentiated and differentiated F9 cell lysates (Knight, A. S., et al. (2009). Oncogene. 28(15):1737-1747.).
Western Blotting Analysis: A representative lot from an independent laboratory in cycling NIH/3T3 and F9 nuclear extracts (Muller, G. A., et al. (2012). Nucleic Acids Res. 40(4):1561-78.).
Western Blotting Analysis: A representative lot from an independent laboratory detected B-Myb wild-type cycling MEF cell lysates and not in B-Myb wild type G0 phase arrested MEF cell lysates. The same lot also detected B-Myb in G0 phase arrested and in cycling MEF cell lysates inappropriately expressing homozygous mutant B-Myb (Tavner, F., et al. (2007). Oncogene. 26(19): 2727-2735.).
质量
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 1 μg/mL of this antibody detected B-Myb in 10 μg of HEK293 cell lysate.
Western Blotting Analysis: 1 μg/mL of this antibody detected B-Myb in 10 μg of HEK293 cell lysate.
目标描述
~80 kDa observed
外形
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Ruchi Kumari et al.
Scientific reports, 11(1), 21506-21506 (2021-11-04)
Cellular senescence is a stable cell cycle arrest that normal cells undergo after a finite number of divisions, in response to a variety of intrinsic and extrinsic stimuli. Although senescence is largely established and maintained by the p53/p21WAF1/CIP1 and pRB/p16INK4A
Benjamin B Morris et al.
Frontiers in oncology, 10, 585551-585551 (2021-01-26)
It has long been recognized that defects in cell cycle checkpoint and DNA repair pathways give rise to genomic instability, tumor heterogeneity, and metastasis. Despite this knowledge, the transcription factor-mediated gene expression programs that enable survival and proliferation in the
Pirunthan Perampalam et al.
The Journal of clinical investigation, 131(4) (2021-01-15)
DREAM (Dp, Rb-like, E2F, and MuvB) is a transcriptional repressor complex that regulates cell proliferation, and its loss causes neonatal lethality in mice. To investigate DREAM function in adult mice, we used an assembly-defective p107 protein and conditional deletion of
David Gallo et al.
Nature, 604(7907), 749-756 (2022-04-22)
Amplification of the CCNE1 locus on chromosome 19q12 is prevalent in multiple tumour types, particularly in high-grade serous ovarian cancer, uterine tumours and gastro-oesophageal cancers, where high cyclin E levels are associated with genome instability, whole-genome doubling and resistance to
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