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生物来源
mouse
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
E4, monoclonal
种属反应性
human
技术
western blot: suitable
同位素/亚型
IgG2bκ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
acetylation (not specified)
基因信息
human ... PARP1(142)
一般描述
Poly (ADP-ribose) polymerase 1 (PARP1) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair. As a marker for apoptosis, PARP is cleaved in vitro by many caspases and in vivo by Caspase-3. Acetylation of PARP1 by p300/CREB-binding protein is important in the regulation of NF-kappaB-dependent gene activation through enhanced functional interactions with p300 and the Mediator complex.
免疫原
Linear peptide corresponding to acetylated human PARP1, short fragment.
应用
Anti-acetyl PARP1 Antibody, clone E4 is a highly specific mouse monoclonal antibody & that targets PARP & has been tested in western blotting.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected wild type acetyl-PARP 1, but did not react with four acetylation-deficient PARP1 KQR mutants in WB (Messner, S., et al. (2009). The FASEB Journal. 23:3978-3989).
质量
Evaluated by Western Blotting in acetylated PARP1 fragment (373-529) purified protein lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected acetyl PARP1 in 10 µg of acetylated PARP1 fragment (373-529) purified protein lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected acetyl PARP1 in 10 µg of acetylated PARP1 fragment (373-529) purified protein lysate.
目标描述
~ 45 kDa observed. Both proteins we provided were acetylated in vitro by p300 using acetyl-CoA. While the short fragment (bacterially expressed) is very nicely acetylated, the full-length (bacolu system) is only weakly modified. The detection of the full-length protein is thus much more difficult.
外形
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Acetylated PARP1 fragment (373-529) purified protein lysate
Acetylated PARP1 fragment (373-529) purified protein lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Experimental and therapeutic medicine, 15(4), 3509-3515 (2018-03-17)
The present study aimed to investigate the effects of JWA knockout (JWA-/-) on malignant transformation of murine embryonic fibroblast (MEF) cells using a conditional JWA-/- mouse model. Once MEF cells were prepared, the potential role of JWA-/- on proliferation, migration
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