产品名称
抗-嘌呤霉素,克隆12D10,Alexa Fluor™ 488 偶联抗体, clone 12D10, 0.5 mg/mL, from mouse
Quality Level
biological source
mouse
conjugate
ALEXA FLUOR™ 488
antibody form
purified antibody
antibody product type
primary antibodies
clone
12D10, monoclonal
species reactivity
human
concentration
0.5 mg/mL
technique(s)
immunocytochemistry: suitable
shipped in
wet ice
target post-translational modification
unmodified
Biochem/physiol Actions
该抗体可检测掺入嘌呤霉素的新合成蛋白质。
证明可与嘌呤霉素预孵育的人类检测样品反应。当检测样品与嘌呤霉素一起孵育时,预计会与所有物种发生反应。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Analysis Note
通过免疫细胞化学在未处理和嘌呤霉素处理的HeLa细胞中进行评估。
免疫细胞化学分析:该抗体的 1:2,500 稀释液可在仅用嘌呤霉素处理的 HeLa 细胞中检测到掺入嘌呤霉素的新合成蛋白质。
Alexa Fluor™ 是 Life Technologies 的注册商标。
免疫细胞化学分析:该抗体的 1:2,500 稀释液可在仅用嘌呤霉素处理的 HeLa 细胞中检测到掺入嘌呤霉素的新合成蛋白质。
Alexa Fluor™ 是 Life Technologies 的注册商标。
Application
研究子类别
细胞周期,DNA 复制&修复
细胞周期,DNA 复制&修复
研究类别
表观遗传学&核功能
表观遗传学&核功能
该抗嘌呤霉素克隆 12D10 经过验证,可用于免疫细胞化学检测嘌呤霉素。
General description
嘌呤霉素是一种氨基核苷酸抗生素,来源于白黑链霉菌,是蛋白合成抑制剂,可通过核糖体中的过早链终止来阻止翻译。抗嘌呤霉素的单克隆抗体可与标准免疫化学方法一起用于直接监测翻译,这种方法称为翻译表面感应(SUnSET)。该分子的一部分类似于氨基酰化tRNA的3′端,这使其可用于蛋白质翻译分析。嘌呤霉素可诱导胸腺细胞和人 HL-60 白血病细胞中的 DNA 断裂。
该产品是货号 MABE343 的缀合版本。
该产品是货号 MABE343 的缀合版本。
请参阅货号 MABE343 用于观察分子量信息。
Immunogen
掺入嘌呤霉素的多种分子量的新合成蛋白质
Physical form
纯化的大鼠单克隆缀合物在含有 PBS 和 15 mg/ml BSA 以及 0.1% 叠氮化钠的缓冲液中。
蛋白G纯化
Preparation Note
自收到之日起,在2-8°C条件下可稳定保存1年。
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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