生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
EA10, monoclonal
种属反应性
human
技术
flow cytometry: suitable
western blot: suitable
同位素/亚型
IgG1κ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... CDKN1A(1026)
相关类别
一般描述
The tumor suppressor p53 transcriptionally activates a number of genes including the WAF1/CIP1 gene in response to DNA damage. The ~21 kDa product of the WAF1 gene is found in a complex involving cyclins, CDKs, and PCNA in normal cells but not transformed cells and appears to be a universal inhibitor of CDK activity. One consequence of p21WAF1 binding to and inhibiting CDKs is the prevention of CDK-dependent phosphorylation and subsequent inactivation of the Rb protein which is essential for cell cycle progression. p21WAF1 is, therefore, a potent and reversible inhibitor of cell cycle progression at both the G1 and G2 checkpoints, presumably to allow sufficient time for DNA repair to be completed. Irreversible G1 or G2 arrest leads to apoptosis. While the role of p21WAF1 in apoptosis is less clear, it is known that p53-mediated apoptosis leads to increased WAF1 expression. Induction of p21WAF1 can occur by both p53-dependent and p53-independent mechanisms, in response to certain observed conditions. p21WAF1 has also been identified as a gene involved in cellular senescence, termed sdi1. Its overexpression was observed to inhibit cellular growth.
免疫原
Recombinant protein corresponding to human p21WAF1.
应用
Anti-p21WAF1 Antibody, clone EA10 is a Mouse Monoclonal Antibody for detection of p21WAF1 & has been validated in WB, FC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Western Blot Analysis: A representative lot from an independent laboratory detected p21WAF1 in non-irradiated and gamma irradiated CLL cells (Carter, A., et al. (2004). Br J Haematol. 127(4):425-428).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected p21WAF1 in normal human lower back tissue (el-Deiry, W. S., et al. (1995). Cancer Res. 55(13):2910-2919.).
Flow Cytometry Analysis: A representative lot from an independent laboratory detected p21WAF1 in non-irradiated and gamma irradiated CLL cells (Carter, A., et al. (2004). Br J Haematol. 127(4):425-428).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected p21WAF1 in normal human lower back tissue (el-Deiry, W. S., et al. (1995). Cancer Res. 55(13):2910-2919.).
Flow Cytometry Analysis: A representative lot from an independent laboratory detected p21WAF1 in non-irradiated and gamma irradiated CLL cells (Carter, A., et al. (2004). Br J Haematol. 127(4):425-428).
质量
Evaluated by Western Blot in HT-29 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected p21WAF1 in 10 µg of HT-29 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected p21WAF1 in 10 µg of HT-29 cell lysate.
目标描述
~21 kDa observed
外形
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
HT-29 cell lysate
HT-29 cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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