推荐产品
生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
K121, monoclonal
种属反应性
human, mouse
技术
affinity chromatography: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
同位素/亚型
IgG1κ
运输
wet ice
靶向翻译后修饰
unmodified
一般描述
The 2,2,7-trimethylguanosine cap structure is found on the 5’ end of small nuclear RNAs and small nucleolar RNAs. Initially, these RNA molecules are capped with 7-monomethylguanosine (m7G) which is then methylated by the trimethylguanosine synthase enzyme to form 2,2,7-trimethylguanosine. The synthesis of 2,2,7-trimethylguanosine occurs in the cytoplasm and is an important marker for nuclear localization of RNA molecules. Capping of RNAs is an important requirement for RNA transport and splicing processes. Uncapped RNAs are rapidly degraded by the 5′ exoribonuclease enzyme. 2,2,7-trimethylguanosine capping may play a role in the expression of viral RNAs.
应用
Anti-2, 2, 7-Trimethylguanosine Antibody, clone K121 is a high quality Mouse Monoclonal Antibody for the detection of 2, 2, 7-Trimethylguanosine & has been validated in ICC, PAI & IP.
Immunoaffinity Purification Analysis: A representative lot from an independent laboratory was used in IAP (Krainer, A.R., et al. (1988). Nucleic Acids Res. 16(20):9415-9429.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory was immunoprecipitated in IP (Moketi, S., et al. (2002). Mol Cell. 10(3):599-609.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory was immunoprecipitated in IP (Moketi, S., et al. (2002). Mol Cell. 10(3):599-609.).
质量
Evaluated by Immunocytochemistry in NIH/3T3, HeLa, and A431 cells.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected 2,2,7-trimethylguanosine in NIH/3T3, HeLa, and A431 cells.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected 2,2,7-trimethylguanosine in NIH/3T3, HeLa, and A431 cells.
外形
Format: Purified
分析说明
Control
NIH/3T3, HeLa, and A431 cells
NIH/3T3, HeLa, and A431 cells
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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