MABE285
抗复制蛋白 A 抗体，克隆 RPA34-20
clone RPA34-20, from mouse
别名:
Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, RF-A protein 2, Replication protein A 34 kDa subunit, RP-A p34
产品名称
抗复制蛋白 A 抗体，克隆 RPA34-20, clone RPA34-20, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RPA34-20, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... RPA2(6118)
Analysis Note
对照
HeLa 细胞裂解液.B10
HeLa 细胞裂解液.B10
通过蛋白质印迹法在HeLa细胞裂解液中进行了评估。
蛋白质印迹分析：1 µg/mL 的该抗体可检测 10 µg HeLa 细胞裂解物中的复制蛋白 A。
蛋白质印迹分析：1 µg/mL 的该抗体可检测 10 µg HeLa 细胞裂解物中的复制蛋白 A。
Application
使用经 WB、ICC、IHC 验证的抗复制蛋白 A 抗体、克隆 RPA34-20（小鼠单克隆抗体）来检测复制蛋白 A，也称为复制蛋白 A 32 kDa 亚基、RP-A p32。
免疫细胞化学分析：代表性批次的 1:500 稀释液在 HeLa 和 A431 细胞中检测到复制蛋白 A。
免疫组织化学分析：代表性批次的 1:5 稀释液在人结直肠腺癌组织中检测到复制蛋白 A。
免疫组织化学分析：代表性批次的 1:5 稀释液在人结直肠腺癌组织中检测到复制蛋白 A。
研究子类别
细胞周期，DNA 复制&修复
细胞周期，DNA 复制&修复
研究类别
表观遗传学&核功能
表观遗传学&核功能
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
RPA (RP-A p32) 是一种异源三聚体蛋白复合物，可特异性结合单链 DNA (ssDNA)。它由三个亚基组成：RPA1 (70 kDa)、RPA2 (32 kDa) 和 RPA3 (14 kDa)。RPA 在 DNA 复制中发挥多种作用。DNA 复制开始时，RPA 被加载到染色质上，随后加载 DNA 聚合酶和其他复制蛋白来启动 DNA 复制。复制开始后，RPA 随复制叉移动，稳定 ssDNA 并协助 DNA 合成。除了其复制功能外，RPA 还在损伤修复和重组中发挥重要作用。当细胞进入 S 期时，32 kDa 亚基被 cdc2 激酶家族磷酸化；以及因响应 DNA 损伤而被 ATM、ATR 和 DNA-PK 蛋白磷酸化。
观测分子量〜30 kDa。Uniprot 描述了 ~29 kDa、~30 kDa 和 ~39 kDa 的三种亚型
Immunogen
从 U293 细胞中纯化的复制蛋白 A
Other Notes
替代：NA19L
浓度：请参考批次特异性浓缩物的检验报告。
Physical form
形式：纯化
纯化的小鼠单克隆IgG1κ，溶于含0.1 M Tris-甘氨酸（pH 7.4），150 mM NaCl和0.05％叠氮化钠的缓冲液中。
蛋白G纯化
Preparation Note
自收到之日起，在2-8°C条件下可稳定保存1年。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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