Anti-ZFX Antibody, clone 3E8.1

ZFX, also known as Zinc finger X-chromosomal protein, and encoded by the gene name ZFX, is probable transcriptional activator. This gene on the X chromosome is structurally similar to a related gene on the Y chromosome. It encodes a member of the krueppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD), a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types, but it was dispensable for growth and differentiation of their progeny. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, but the full-length nature of some variants has not been determined.

GST-tagged recombinant protein corresponding to human ZFX.

Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected ZFX HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-ZFX antibody is validated for use in WB, IC for the detection of ZFX.

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ZFX in 200 µg of HeLa nuclear extract.

~130, ~90, ~59 kDa observed. Three bands (~130 kDa, ~91 kDa and ~59 kDa) corresponding to ZFX were detected. The ~130 kDa band is nuclear, while the ~91 kDa band was found in the cytoplasmic (Zhu, Z., et al. (2013). J Neurooncol. 112:17-25).

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.