推荐产品
生物来源
rat
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
AB3/63.3, monoclonal
种属反应性(根据同源性预测)
all
技术
ChIP: suitable
dot blot: suitable
immunocytochemistry: suitable
同位素/亚型
IgG2aκ
运输
wet ice
靶向翻译后修饰
unmodified
一般描述
Four types of cytosine modifications have been identified in mammalian DNA, namely 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). While 5mC, 5hmC, and 5fC occur in embryonic stem (ES) cells and many cell types in all the major organs (e.g. brain, heart, lung and kidney), 5caC has only been detected in a few cell types at a 10 times lower level than 5fC. The sequential oxidation of 5mC to 5hmC, 5fC, and 5caC catalyzed by the ten-eleven translocation (TET) enzymes has been proposed as a potential route for the demethylation of 5mC back to cytosine (C). Thymine DNA glycosylase (TDG), for example, can excise 5fC and 5caC from DNA as part of the base excision repair (BER) pathway to restore the C-nucleotide. Emerging evidences suggest that the different modifications may be selectively recognized by various proteins, and methyl-CpG-binding protein 2 (MeCP2) binding to 5hmC has been proposed as a means of epigenetic regulation in neurons. The conversion of 5hmC to 5fC, in turn, induces an enhanced binding of DNA-repair-associated proteins to 5fC, which has been proposed as a signal for DNA demethylation.
特异性
Target modification is not species-specific.
This antibody detects 5-hydroxymethylcytosine, but not 5-methylcytosine or unmethylated cytosine.
免疫原
5-Hydroxymethylcytosine conjugated to BSA
应用
Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone AB3/63.3 is a rat monoclonal antibody that detects 5hmC modification and has been validated in Dot Blot, Immunocytochemistry, and Chromatin Immunoprecipitation (ChIP).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected 5-hydroxymethylcytosine (5hmC) enrichment sites using liver chromatin preparations from wild-type female mice and mice with liver-specific Hnf4a knockout. A lower 5hmC modification level at Slc22a6 exon1 and Mgst3 intron1–2 was found associated with Hnf4a deficiency (Zhang, Q., et al. (2014). PLoS One. 9(1):e84925).
Immunocytochemistry Analysis: A representative lot detected embryogenesis stage-dependent 5hmC immunoreactivity by indirect fluorescent staining of 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized, 2N HCl-DNA denatured porcine iPS and advanced pronuclear embryos of different origins (Cao, Z., et al. (2014). Theriogenology. 81(3):496-508).
Immunocytochemistry Analysis: A representative lot detected embryogenesis stage-dependent 5hmC immunoreactivity by indirect fluorescent staining of 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized, 2N HCl-DNA denatured porcine iPS and advanced pronuclear embryos of different origins (Cao, Z., et al. (2014). Theriogenology. 81(3):496-508).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
质量
Evaluated by Dot Blot in a panel of of DNA cytosine modifications.
Dot Blot Analysis: 1-10 µg/mL of this antibody detected 10-50 ng of DNA with 5-hydroxymethylcytosine (5hmC) modification, but not DNA with only unmodified cytosine or 5-methylcytosine (5mC) modifiction.
Dot Blot Analysis: 1-10 µg/mL of this antibody detected 10-50 ng of DNA with 5-hydroxymethylcytosine (5hmC) modification, but not DNA with only unmodified cytosine or 5-methylcytosine (5mC) modifiction.
目标描述
Variable, depending on the size(s) of the DNA molecule(s) with 5-hydroxymethylcytosine (5hmC) modification.
外形
Protein G Purified
Format: Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA
Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
PloS one, 9(1), e84925-e84925 (2014-01-16)
Hepatocyte nuclear factor 4α (HNF4α) is a liver-enriched transcription factor essential for liver development and function. In hepatocytes, HNF4α regulates a large number of genes important for nutrient/xenobiotic metabolism and cell differentiation and proliferation. Currently, little is known about the
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门