推荐产品
生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
15G8.1, monoclonal
种属反应性
human, mouse
技术
immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型
IgG2aκ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... KDM5B(10765)
一般描述
Lysine-specific demethylase 5B (UniProt Q9UGL1; also known as Cancer/testis antigen 31, CT31, Histone demethylase JARID1B, Jumonji AT rich interactive domain 1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Protein phosphatase 1 regulatory subunit 98, Putative DNA/chromatin binding motif, RBP2-H1, Retinoblastoma-binding protein 2 homolog 1) is encoded by the KDM5B (also known as PLU1, PUT1, JARID1B, PPP1R98, RBBP2H1, RBBP2H1A) gene (Gene ID 10765) in human. JARID1B/KDM5B is an H3K4me2/3 demethylase that regulates self-renewal and differentiation in a cell type-specific manner. JARID1B/KDM5B is highly expressed in embryonic stem cells (ESCs), hematopoietic stem cells (HSCs), and in various primary cancers including melanoma, breast, testicular, and is upregulated in leukemic cell lines.
免疫原
Epitope: Region between ARID and JmjC domain
GST-tagged recombinant protein corresponding to the region between ARID and JmjC domain of human KDM5B.
应用
This Anti-KDM5B Antibody, clone 15G8.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of KDM5B.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected KDM5B in 10 µg of MCF-7 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected KDM5B in human kidney and mouse cerebral cortex tissue.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected KDM5B in human kidney and mouse cerebral cortex tissue.
质量
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected KDM5B in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected KDM5B in 10 µg of HeLa cell lysate.
目标描述
~175 kDa observed. Uncharacterized band(s) may appear in some lysates.
外形
Format: Purified
其他说明
Concentration: Please refer to lot specific datasheet.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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