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生物来源
rat
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
5F11, monoclonal
种属反应性
mouse
技术
immunocytochemistry: suitable
western blot: suitable
同位素/亚型
IgG2aκ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
mouse ... Myod1(17927)
一般描述
Myoblast determination protein 1 (MyoD1) is a myogenic transcription factor required for the growth, repair, and regeneration of muscle cells. It induces differentiation of myoblast cells into multinucleated myotubules, activating genes and proteins involved in cell cycle arrest, and differentiation. MyoD1 may also control proliferation and homeostasis during embryogenesis, in the absence of Myf5.
免疫原
GST-tagged recombinant protein corresponding to mouse MyoD.
应用
Detect MyoD using this Anti-MyoD Antibody, clone 5F11 validated for use in Western Blotting, ICC.
Immunocytochemistry Analysis: A representative lot detected MyoD in C2C12 cells.
Western Blot Analysis: A representative lot detected MyoD in C2C12 cells.
Western Blot Analysis: A representative lot detected MyoD in C2C12 cells.
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
质量
Evaluated by Western Blot in C2C12 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected MyoD in 10 µg of C2C12 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected MyoD in 10 µg of C2C12 cell lysate.
目标描述
~45 kDa observed. An uncharacterized band at ~38 kDa may be observed in some cell lysates. The calculated molecular weight is 34 kDa; however, this protein is known to be glycosylated.
联系
Replaces: MABE514
外形
Protein G Purified
Format: Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
C2C12 cell lysate
C2C12 cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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