Anti-TAF10/TAFII30 Antibody, clone 6TA-2B11

Transcription initiation factor TFIID subunit 10 (UniProt Q12962; also known as STAF28, TAF(II)30, TAFII-30, TAFII30, Transcription initiation factor TFIID 30 kDa subunit) is encoded by the TAF10 (also known as TAF2A, TAF2H, TAFII30) gene (Gene ID 6881) in human.Transcription initiation factor TFIID subunit 10 (UniProt Q12962; also known as STAF28, TAF(II)30, TAFII-30, TAFII30, Transcription initiation factor TFIID 30 kDa subunit) is encoded by the TAF10 (also known as TAF2A, TAF2H, TAFII30) gene (Gene ID 6881) in human. TAF10 (TAFII30) is a subunit of both the general transcription factor (GTF) TFIID and the coactivator SAGA (Spt-Ada-Gcn5-acetyltransferase) histone acetyltransferase (HAT) complexes involved in the initiation of RNA polymerase II (Pol II) transcription in eukaryotes. TFIID complex is composed of the TATA-binding protein (TBP) and thirteen TBP-associated factors (TAFs) that allow its recognition of not only TATA box-containing, but also TATA-less promoters for site-specific assembly of preinitiation complex (PIC). TAF10-knockout studies in mice indicate that TAF10 plays an important role during embryonic development and homeostasis in a tissue-dependent manner. TAF10 ablation is reported to result in severe anemia and lethality at embryonic stage E13.5 due to impaired erythropoiesis. In addition, TAF10 is shown to not only interact with the transcription factor GATA1, but also regulate its transcription as evidenced by its occupancy at GATA1 genomic locus in human fetal erythroid cells.

Clone 6TA-2B11 (a.k.a. 6TA 2B11) specifically immunostained wild-type, but not Taf10-/-, mouse embryonic blastocysts (Mohan, W.S. Jr, et al. (2003). Mol .Cell. Biol. 23(12):4307-4318).

Epitope: Internal (N-terminal half).

Recombinant human TAF10/TAFII30.

Anti-TAF10Antibody, clone 6TA-2B11 is an antibody against TAFII30 for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.

Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected TAF10/TAFII30 in A431 and HeLa cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TAF10/TAFII30 in TAFIID complex isolated from HeLa nuclear extract (Courtesy of Dr. Mustapha OULAD-ABDELGHANI, IGBMC, France).
Immunoprecipitation Analysis: A representative lot immunoprecipitated TAF10 together with associated GATA1 from murine erythroleukemia (MEL) cell nuclear extracts (Papadopoulos, P., et al. (2015). Mol. Cell. Biol. 35(12):2103-2118).
Western Blotting Analysis: A representative lot detected TAF10 in E12.5 mouse fetal liver nuclear extracts and murine erythroleukemia (MEL) cell nuclear extracts, as well as in GATA1 immunoprecipitate from MEL cell and fetal liver nuclear extracts (Papadopoulos, P., et al. (2015). Mol. Cell. Biol. 35(12):2103-2118).
Immunocytochemistry Analysis: A representative lot detected TAF10 immunoreactivity in trophoblasts from 3-day cultured wild-type, but not Taf10-/-mouse embryonic blastocysts isolated at 3.5 dpc (days post-coitum) by immunofluorescent staining of 1% paraformaldehyde-fixed, 0.1% Tween 20-permeabilized blastocysts (Mohan, W.S. Jr, et al. (2003). Mol .Cell. Biol. 23(12):4307-4318).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected TAF10/TAFII30 in 10 µg of A431 cell lysate.

~30 kDa observed. 21.71 kDa calculated.

Unpurified

Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to lot specific datasheet.

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