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安全信息

MABE1031

Sigma-Aldrich

抗-聚ADP核糖结合试剂

Anti-poly-ADP-ribose binding reagent is a reagent that selectively binds to ADP ribose for use in Western Blotting, Immunocytochemistry and Dot Blot.

别名:

poly-ADP-ribose binding reagent

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160405
NACRES:
NA.41

生物来源

Escherichia coli

质量水平

抗体形式

purified antibody

抗体产品类型

primary antibodies

种属反应性

human, mouse

种属反应性(根据同源性预测)

all

技术

dot blot: suitable
immunocytochemistry: suitable
western blot: suitable

运输

dry ice

靶向翻译后修饰

unmodified

相关类别

一般描述

抗聚ADP-核糖结合试剂(货号MABE1031)是一种与兔Fc标记融合的His标记重组蛋白,通过大肠杆菌的Rosetta(DE3)pLysS菌株(货号70956)表达和纯化。抗聚ADP-核糖结合试剂可用于亲和检测检测膜上或固定细胞中的寡-和聚ADP-核糖(PARylated)蛋白,类似于抗体法蛋白质印迹、斑点印迹和免疫细胞化学应用。兔Fc标记可使偶联的抗兔二抗的结合/标记成像。Fc标记还可让抗-聚ADP-核糖结合试剂捕获在蛋白A树脂上,适用于亲和pull-down 应用。

特异性

两个或更多的ADP核糖单元

应用

抗聚ADP核糖结合试剂是一种可选择性结合ADP核糖的试剂,适用于蛋白质印迹、免疫细胞化学和斑点印迹。
斑点印迹特异性分析:该试剂可检测 ADP核糖化的重组PARP1蛋白上的寡(ADPR)和聚(ADPR)(Lee Kraus,University of Texas Southwestern Medical Center)。
免疫细胞化学分析:一个代表性批次检测到3T3-L1细胞中的寡(ADPR)和聚(ADPR)(由University of Texas Southwestern Medical Center的Lee Kraus提供)。
研究子类别
常规翻译后修改
研究类别
表观遗传学&核功能

质量

通过蛋白质印迹法对ADP-核糖基化的PARP1和PARP3重组蛋白进行评估。

蛋白质印迹分析:该试剂可检测 ADP核糖化重组PARP1蛋白上的寡(ADPR)和聚(ADPR)(Lee Kraus,University of Texas Southwestern Medical Center)。

目标描述

因靶蛋白和ADP-核糖基化程度而异。

外形

Ni-NTA琼脂糖
形式:纯化
通过Ni-NTA琼脂糖从大肠杆菌中纯化。以含10 mM Tris pH 7.5、0.2 M NaC、10%甘油、10 mM咪唑、1 mM PMSF、1 mM β-巯基乙醇、10%甘油,不含防腐剂的缓冲液形式提供。

储存及稳定性

自接收之日起,在-80°C下可稳定保存1年。
处理建议: 收货后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。分装至微量离心管中,并储存于-80°C。避免反复冻融循环,以免损坏IgG和影响产品性能。

其他说明

浓度:请参考特定批次的数据表。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物食用或应用。

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WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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Xin Luo et al.
Molecular cell, 65(2), 260-271 (2017-01-21)
Poly(ADP-ribosyl)ation (PARylation) is a post-translational modification of proteins mediated by PARP family members, such as PARP-1. Although PARylation has been studied extensively, few examples of definitive biological roles for site-specific PARylation have been reported. Here we show that C/EBPβ, a key
George E Ronson et al.
Nature communications, 9(1), 746-746 (2018-02-23)
PARP1 regulates the repair of DNA single-strand breaks generated directly, or during base excision repair (BER). However, the role of PARP2 in these and other repair mechanisms is unknown. Here, we report a requirement for PARP2 in stabilising replication forks
Anna-Lena Kolb et al.
Methods in molecular biology (Clifton, N.J.), 1813, 125-148 (2018-08-12)
The amoeba Dictyostelium discoideum is a single-cell organism that can undergo a simple developmental program, making it an excellent model to study the molecular mechanisms of cell motility, signal transduction, and cell-type differentiation. A variety of human genes that are
Lesley B Conrad et al.
Molecular cancer therapeutics, 19(1), 282-291 (2019-10-09)
Inhibitors of nuclear PARP enzymes (e.g., PARP-1) have improved clinical outcomes in ovarian cancer, especially in patients with BRCA1/2 gene mutations or additional homologous recombination (HR) DNA repair pathway deficiencies. These defects serve as biomarkers for response to PARP inhibitors
Tom P Aird et al.
American journal of physiology. Endocrinology and metabolism, 321(6), E802-E820 (2021-11-09)
Sprint interval training (SIT) is a time-efficient alternative to endurance exercise, conferring beneficial skeletal muscle metabolic adaptations. Current literature has investigated the nutritional regulation of acute and chronic exercise-induced metabolic adaptations in muscle following endurance exercise, principally comparing the impact

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