推荐产品
生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
9B7, monoclonal
种属反应性
mouse
种属反应性(根据同源性预测)
human (immunogen homology)
技术
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型
IgG1κ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... POU5F1(5460)
一般描述
The POU5F1 gene expresses the POU-domain transcription factor, Octamer-4 (Oct-4), which is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.
免疫原
Recombinant protein corresponding to human Oct-4.
应用
Anti-Oct-4 Antibody, clone 9B7 detects level of Oct-4 & has been published & validated for use in WB, IH & IF.
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in human lung tissue.
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in COS7 cells.
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in COS7 cells.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Transcription Factors
Transcription Factors
质量
Evaluated by Western Blot in mouse embryonic stem cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Oct-4 in 10 µg of mouse embryonic stem cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Oct-4 in 10 µg of mouse embryonic stem cell lysate.
目标描述
~39 kDa observed
外形
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Mouse embryonic stem cell lysate
Mouse embryonic stem cell lysate
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Molecular systems biology, 14(9), e8140-e8140 (2018-09-05)
It is well known that clonal cells can make different fate decisions, but it is unclear whether these decisions are determined during, or before, a cell's own lifetime. Here, we engineered an endogenous fluorescent reporter for the pluripotency factor OCT4
Stem cell research, 52, 102238-102238 (2021-02-22)
Two cell lines were generated by CRISPR/Cas9 mediated knockout of MKK7 (MAP2K7) by removal of exon 1 or exons 4 through 7. These knockouts were confirmed at the transcript and protein levels. These hESCs are pluripotent and maintain tri-lineage differentiation
Nature communications, 11(1), 1617-1617 (2020-04-03)
Translational control plays a central role in regulation of gene expression and can lead to significant divergence between mRNA- and protein-abundance. Here, we used genome-wide approaches combined with time-course analysis to measure the mRNA-abundance, mRNA-translation rate and protein expression during
Stem cell reviews and reports, 13(2), 299-308 (2016-12-18)
Stem cells (SCs) are a promising approach to regenerative medicine, with the potential to treat numerous orthopedic disorders, including osteo-degenerative diseases. The development of human-induced pluripotent stem cells (hiPSCs) has increased the potential of SCs for new treatments. However, current
Oncogene, 38(22), 4412-4424 (2019-02-12)
Stem cell characteristics have been associated with treatment resistance and poor prognosis across many cancer types. The ability to induce and regulate the pathways that sustain these characteristic hallmarks of lethal cancers in a novel in vitro model would greatly
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