Anti-SHH Antibody, clone 5H4

Sonic hedgehog protein (SHH) or alternatively known as HHG-1, is a very important developmental intercellular signaling factor. SHH protein is cleaved into to active forms, an N-terminal product that stays associated with the cell membrane or forms freely diffusible multimers and a C-terminal form that diffuses into the extracellular matrix. SHH is expressed in fetal intestine, liver, lung and kidney but not normally in adult tissues except during some disease state like cancerous tumors. The intercellular signaling triggered by SHH is essential for a variety of patterning events during development including the development of the notochord, neural tube and plate, as well as the polarizing of the anterior-posterior axis of developing limb buds. Because of its importance in patterning during development, defects in SHH are the cause of a number of abnormalities including structural eye and brain abnormalities, teeth and bone malformations and finally limb and bone developmental defects. EMD-Millipore’s Anti-SHH mouse monoclonal antibody has been tested in western blot against recombinant antigen and cell lysates from HepG2, PANC-1, HeLa, SK-N-SH, F9, NIH3T3 and COS7 cells and in paraffin embedded immunohistochemistry of human liver cancer tissue sections. The antibody has also been successfully tested by flow cytometry against HeLa cells in culture.

Purified recombinant fragment of human SHH expressed in E. Coli.

Anti-SHH Antibody, clone 5H4 is a highly specific mouse monoclonal antibody, that targets Sonic Hedgehog & has been tested in western blotting, Flow Cytometry & IHC.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected SHH in liver cancer tissue.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected SHH in non serum starved HeLa cells.

Optimal working dilutions must be determined by end user.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Evaluated by Western Blotting in LNCaP, HepG2, PANC-1, HeLa, SK-N-SH, F9, NIH3T3, COS7 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected SHH in LNCaP, HepG2, PANC-1, HeLa, SK-N-SH, F9, NIH3T3, COS7 cell lysates.

~50 kDa observed. Uncharacterized bands may appear in some lysate(s).

Protein G Purified

Format: Purified

Purified mouse monoclonal in PBS with up to 0.1% sodium azide and 0.05% protein stabilizer.

Stable for 1 year at 2-8°C from date of receipt.

Control
LNCaP, HepG2, PANC-1, HeLa, SK-N-SH, F9, NIH3T3, COS7 cell lysates

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.