Anti-DMP1, NT Antibody, clone 9B6.3

Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene.

This antibody recognizes the N-terminus of DMP1.

Epitope: N-terminus

N-terminus fragment of rat DMP1 isolated from long bone.

Detect DMP1, N-terminus using this Anti-DMP1, N-terminus Antibody, clone 9B6.3 validated for use in Western Blotting.

Research Category
Stem Cell Research

Research Sub Category
Endothelial Stem Cells

Evaluated by Western Blot in rat dentin tissue extract.

Western Blot Analysis: 1 µg/mL of this antibody detected DMP1 in 10 µg of rat dentin tissue extract.

~65 kDa observed. Uniprot describes a molecular weight at ~53 kDa Uniprot describes that this protein is highly phosphorylated and highly glycosolated.

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Rat dentin tissue extract

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.