产品名称
抗-TRAF4抗体,克隆5MLN-2H1, ascites fluid, clone 5MLN-2H1, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
5MLN-2H1, monoclonal
species reactivity
mouse, human
species reactivity (predicted by homology)
rat (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TRAF4(9618)
Analysis Note
已通过蛋白质印迹对HeLa细胞裂解液进行了评估。
蛋白质印迹分析:该抗体的1:1000 的稀释液在10 µg HeLa细胞裂解液中检测到TRAF4。
蛋白质印迹分析:该抗体的1:1000 的稀释液在10 µg HeLa细胞裂解液中检测到TRAF4。
Application
免疫组织化学分析:该抗体的代表性批次在多种人类癌症组织中检测到TRAF4(Camilleri-Broet.S., et al. (2007) Oncogene.26:142-147)。
免疫组织化学分析:该抗体的代表性批次在乳腺癌组织中检测到TRAF4(Kedinger, V., et. al. (2008) PLoS ONE. 3(10):e3518)。
免疫细胞化学分析:该抗体的代表性批次在MCF-10A细胞和转染HeLa细胞中检测到TRAF4(Kedinger, V., et. al. (2008) PLoS ONE. 3(10):e3518)。
免疫组织化学分析:该抗体的代表性批次在乳腺癌组织中检测到TRAF4(Kedinger, V., et. al. (2008) PLoS ONE. 3(10):e3518)。
免疫细胞化学分析:该抗体的代表性批次在MCF-10A细胞和转染HeLa细胞中检测到TRAF4(Kedinger, V., et. al. (2008) PLoS ONE. 3(10):e3518)。
研究子类别
肿瘤标记物
肿瘤标记物
研究类别
细胞凋亡 & 癌症
细胞凋亡 & 癌症
该抗-TRAF4抗体(克隆5MLN-2H1)经验证可用于蛋白质印迹、免疫组织化学和免疫细胞化学中TRAF4的检测。
Biochem/physiol Actions
该抗体可检测TRAF4,在出版物中被引用为克隆5MLN-2H1和克隆2H1。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
TNF受体相关因子4(TRAF4),也被称为与RING和Traf结构域相关的半胱氨酸富半胱氨酸结构域蛋白1(CART1)、转移淋巴结基因62蛋白(MLN 62)和环指蛋白83(RNF83),是一种连接TNF家族成员到不同信号通路的适配器蛋白和信号换能器。 因此,TRAF4在NF-κ-和JNK信号的激活中发挥作用,并在细胞凋亡和细胞存活途径中发挥作用。在发育过程中,TRAF4是骨骼和肺正常发育所必需的。TRAF4定位于细胞的核周区域以及其他位置,包括与各种细胞骨架元件相关的细胞连接处。TRAF4在胸腺、呼吸道、唾液腺和食道的上皮细胞中表达。TRAF4的表达受泛素蛋白酶体系统的调控,是一种多泛素靶蛋白。
观测分子量〜53 kDa
Immunogen
对应于人TRAF4的线性肽。
Other Notes
浓度:请参考特定批次的数据表。
Physical form
不含防腐剂的小鼠单克隆IgG1腹水。
未纯化
Preparation Note
自收到之日起在-20°C可稳定保存1年。
使用建议:收货后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。分装到微量离心管中,并储存于-20°C。避免反复冻融循环,以免损坏IgG和影响产品性能。
使用建议:收货后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。分装到微量离心管中,并储存于-20°C。避免反复冻融循环,以免损坏IgG和影响产品性能。
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Ming Li et al.
Cell death & disease, 13(12), 1053-1053 (2022-12-20)
The E3 ligase TNF receptor-associated factor 4 (TRAF4) is frequently overexpressed and closely related to poor prognosis in human malignancies. However, its effect on carcinogenesis and radiosensitivity in oral squamous cell carcinoma (OSCC) remains unclear. The present study found that
Jinzhuang Liao et al.
International journal of biological sciences, 20(1), 182-199 (2024-01-02)
Nasopharyngeal carcinoma (NPC) is the most common cancer originating in the nasopharynx. Despite continuous improvement in treatment strategies, recurrence or persistence of cancer after radiotherapy is still inevitable, highlighting the need to identify therapeutic resistance factors and develop effective methods
Yunxiao Liu et al.
International journal of oncology, 53(4), 1752-1762 (2018-07-18)
Circular RNAs (circRNAs), a class of endogenous RNAs, have emerged as an enigmatic class of genes. However, little is known about their value in the progression and chemoresistance of cancers. The present study sought to determine the expression profiles and
Xin Dong et al.
Cell death & disease, 14(2), 102-102 (2023-02-11)
The E3 ligase TNF receptor-associated factor 4 (TRAF4) is upregulated and closely associated with tumorigenesis and the progression of multiple human malignancies. However, its effect on radiosensitivity in colorectal cancer (CRC) has not been elucidated. The present study found that
Xinfang Yu et al.
Journal of hematology & oncology, 13(1), 40-40 (2020-05-03)
Aberrant activation of DNA damage response (DDR) is a major cause of chemoresistance in colorectal cancer (CRC). CHK1 is upregulated in CRC and contributes to therapeutic resistance. We investigated the upstream signaling pathways governing CHK1 activation in CRC. We identified
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