Anti-Atg18 (WIPI-2) Antibody, clone 2A2

Atg18, also known as WIPI-2, is involved in autophagy by regulating the assembly of mulitprotein complexes by presenting a 7-bladed propeller structure for interactions with phospholipids. Atg18 is a mammalian effector of PtdIns3P and is ubiquitously expressed in a variety of cell lines. Studies show that Atg18 is recruited to early autophagosomal structural formation along with Atg16 and ULK1, and Atg18 is required for the formation of LC3-positive autophagosomes.

This antibody recognizes Atg18 at the C-terminus.

Epitope: C-terminus

Linear peptide corresponding to the C-terminus of human Atg18.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Atg18 in human colorectal adenocarcinoma tissue.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Atg18 (WIPI-2) Antibody, clone 2A2 is validated for use in WB, IH(P) for the detection of Atg18 (WIPI-2).

Evaluated by Western Blot in human testis tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Atg18 in 10 µg of human testis tissue lysate.

~49 kDa observed.
6 isoforms exist with molecular weights ranging from 42 kDa to 48 kDa

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Human testis tissue lysate

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