生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
5C6.2, monoclonal
种属反应性
human
技术
immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型
IgG2bκ
NCBI登记号
UniProt登记号
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
human ... SERPINB5(5268)
一般描述
Serpin B5 (UniProt P36952; also known as Maspin, Peptidase inhibitor 5, PI-5) is encoded by the SERPINB5 (also known as PI5) gene (Gene ID 5268) in human. Serpin B5/Maspin is an evolutionarily conserved serine protease inhibitor that induces apoptosis and inhibits the motility of breast cancer cells. DNA hypermethylation and histone deacetylation lead to silencing of the SERPINB5 gene in human breast cancer.
特异性
Does not detect spliced isoform 2 (UniProt P36952-2).
免疫原
Epitope: C-terminal half
GST-tagged recombinant protein corresponding to the C-terminal of human SERPINB5.
应用
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-SERPINB5 Antibody, clone 5C6.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of SERPINB5.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected SERPINB5 in 10 µg of HT-1080 and HT-29 cell lysates.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected SERPINB5 in human breast tissue.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected SERPINB5 in human breast tissue.
质量
Evaluated by Western Blotting in HCT116 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected SERPINB5 in 10 µg of HCT116 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected SERPINB5 in 10 µg of HCT116 cell lysate.
目标描述
~42 kDa observed
外形
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Cell communication and signaling : CCS, 19(1), 86-86 (2021-08-16)
Maspin (SERPINB5) is a potential tumor suppressor gene with pleiotropic biological activities, including regulation of cell proliferation, death, adhesion, migration and gene expression. Several studies indicate that nuclear localization is essential for maspin tumor suppression activity. We have previously shown
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