Anti-Nucleolin Antibody, clone 364-5-10-5

Nucleolin (UniProt: P19338; also known as Protein C23) is encoded by the NCL gene (Gene ID: 4691) in human. Nucleolin is a highly conserved, multi-functional, phosphoprotein that is ubiquitously distributed in the nucleolus, nucleus and cytoplasm. It is associated with intranucleolar chromatin and pre-ribosomal particles. In the cytoplasm, it is localized in cytoplasmic mRNP granules containing untranslated mRNA. Its levels are correlated with the rate of functional activity of the nucleolus in exponentially growing cells. Nucleolin contains three structural and multifunctional domains: An N-terminal portion containing several acidic stretches; two to four RNA-binding domains known as RNA recognition motifs (RRM) in the central region; and a glycine/arginine-rich domain at the C-terminus. The N-terminal acidic and basic region and the C-terminal domain rich in glycine/arginine repeats are reported to mediate protein-protein interactions with histone H1, U3 snoRNP and ribosomal proteins. Nucleolin contains intrinsic DNA and RNA helicase, nucleic-acid-dependent ATPase, and self-cleaving activities. It is known to bind RNA through its RRMs. It binds RNA oligonucleotides with 5′-UUAGGG-3′ repeats more tightly than the telomeric single-stranded DNA 5′-TTAGGG-3′ repeats. Nucleolin participates in regulation of various aspects of DNA and RNA metabolism, chromatin structure, rDNA transcription, rRNA maturation, cytokinesis, nucleogenesis, cell proliferation and growth. It is also involved in the folding, maturation and ribosome assembly, and nucleocytoplasmic transport of newly synthesized pre-RNAs, gene silencing and senescence. The phosphorylation state of nucleolin is highly regulated during the cell cycle. Extensive phosphorylation by casein kinase 2 is shown to occur at interphase and by CDC2 during mitosis. (Ref.: Abdelmohsen, K., and Gorospe, M. (2012). RNA Biol. 9(6); 799-808; Tajrishi, MM., et al. (2011). Commun. Integr. Biol. 4(3); 267-275).

Clone 364-5-10-5 is a mouse monoclonal antibody that detects Nucleolin.

Nuclei from SU-DHL-1 human lymphoma cells.

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Nucleolin in HeLa cell lysates.

Tested Applications

Immunohistochemistry Applications: A 1:250-1,000 dilution from a representative lot detected Nucleolin in human breast cancer and human kidney tissue sections.

Immunohistochemistry Applications: A 1:10 dilution from a representative lot detected Nucleolin in ovarian cancer metastasis-infiltrated human lymph node tissue.

Flow Cytometry Analysis: A representative lot detected Nucleolin in NK-92MIO cells gated and ungated for CD56.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Nucleolin in A431 and HEK293 cells

Western Blotting Analysis: A representative lot detected Nucleolin in Western Blotting applications. (Kour, R., et al. (2019). RNA. 25(10):1377-1392).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Nucleolin, clone 364-5-10-5, Cat. No. MABC587-I, is a mouse monoclonal antibody that detects Nucleolin and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.